Results 21 to 30 of about 102,030 (267)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Connections between coupling and Ishii-Lions methods for tug-of-war with noise stochastic games
We present a streamlined account of two different regularity methods as well as their connections. We consider the coupling method in the context of tug-of-war with noise stochastic games, and consider viscosity solutions of the p-Laplace equation in the
Anttila Riku +2 more
doaj +1 more source
On the Tonelli method for the degenerate parabolic Cauchy problem with functional argument [PDF]
The degenerate parabolic Cauchy problem is considered. A functional argument in the equation is of the Hale type. As a limit of piecewise classical solutions we obtain a viscosity solution of the main problem. Presented method is an adaptation of Tonelli'
Krzysztof A. Topolski
doaj +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Rheology of Polyhydroxyalkanoate Solutions [PDF]
The rheology of solutions of polyhydroxyalkanoates in chloroform and dichloromethane was studied. The dynamic viscosity of solutions depended on type of solvent and PHA.
Dmitry B. Goncharov, Alexey G. Sukovatyi
doaj
Discerning protein pools by selective staining with self‐labeling tags
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source
Asymptotic mean-value formulas for solutions of general second-order elliptic equations
We obtain asymptotic mean-value formulas for solutions of second-order elliptic equations. Our approach is very flexible and allows us to consider several families of operators obtained as an infimum, a supremum, or a combination of both infimum and ...
Blanc Pablo +3 more
doaj +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source

