Results 101 to 110 of about 418,009 (261)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Analysing epileptic EEGs with a visibility graph algorithm [PDF]
, 2012 This paper analyzes the human epileptic electroencephalogram (EEG) based on a visibility graph algorithm. A single-channel EEG is mapped into a visibility graph (VG). Then its mean degree and degree distribution on the VG are extracted.
Zhu, Guohun +6 more
core +1 more source
Journal of Transport and Supply Chain Management, 2016 Background: Supply chain integration was identified as a tool to assist an organisation to survive in a competitive marketplace. It was unclear how plastic manufacturers in Gauteng perceived supply chain integration efforts between themselves and their ...
Yvonne Vermeulen +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
A New Methodology for Fiscal Visibility of Territorial Government Levels [PDF]
The size and pattern of any public budget depend, among other factors, on the visibility of both the burdens and benefits of public revenue and expenditure.
Miguel Roig-Alonso
core
Interactive global illumination based on coherent surface shadow maps. [PDF]
, 2008 Interactive rendering of global illumination effects is a challenging problem. While precomputed radiance transfer (PRT) is able to render such effects in real time the geometry is generally assumed static.
Seidel, H-P +11 more
core
Journal of Civil Engineering and Management, 2009 The time taken to evacuate space inside a building (required time for evacuation) must be shorter than the time for the environment in that space to become life threatening (available safe egress time ‐ ASET).
Marek Konecki, Marzena Półka
doaj +1 more source
FEBS Open Bio, EarlyView.Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Cyclist visibility at night : perceptions of visibility do\ud not necessarily match reality [PDF]
, 2010 Visibility limitations make cycling at night particularly dangerous. We previously reported cyclists’ perceptions of their own visibility at night and identified clothing configurations that made them feel visible.
Tyrrell, Richard A. +6 more
core
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source