Results 131 to 140 of about 4,066,800 (255)
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Visible-Light-Promoted Unsymmetrical Phosphine Synthesis from Benzylamines
Herein, by applying visible-light photoredox catalysis, we have achieved the catalytic deaminative alkylation of diphenylphosphine and phenyl phosphine with benzylamine-derived Katritzky salts at room temperature.
Xianjin Wang (380110) +5 more
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Norramon Charoenpipatsin,1 Punyanut Yothachai,1 Nuttaporn Nuntawisuttiwong,1 Ornnicha Wongpraparut,2 Pranomkorn Choosri,3 Narumol Silpa-archa1 1Photodermatology Unit, Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University ...
Charoenpipatsin N +5 more
doaj
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source
Visible-Light-Induced Direct Difluoroalkylation of Uracils, Pyridinones, and Coumarins
An efficient and general method for the synthesis of difluoroalkylated uracils, pyridinones, and coumarins through visible-light-induced reaction with commercial materials is developed.
Jingjing Kong (2322235) +5 more
core +1 more source
All-Visible-Light-Activated Diarylethene Photoswitches
Photochromic compounds have attracted much attention for their potential applications in photo-actuators, optoelectronic devices and optical recording techniques.
Ruiji Li +6 more
doaj +1 more source

