Results 81 to 90 of about 1,748,226 (269)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Contextual Graph Modeling for Open-Set Object Detection in Multi-View Autonomous Driving

open access: yesIEEE Open Journal of Vehicular Technology
Autonomous vehicles require robust perception systems capable of accurately identifying both known and unknown objects in complex driving environments. Traditional closed-set detectors risk safety by failing to recognize novel or out-of-distribution (OOD)
Muhammad Asad   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Agentic AI System for Stress Monitoring: A Multi-agent Healthcare Crew

open access: yesInternational Journal of Computational Intelligence Systems
Chronic stress poses serious health risks, but traditional detection methods like self-reporting or infrequent assessments lack timeliness and personalisation.
Talha Iqbal, Edward Curry, Ihsan Ullah
doaj   +1 more source

Information Characteristics and Social Media Information Sharing: Insights From Elaboration Likelihood Model (ELM) for Misinformation Mitigation

open access: yesIEEE Access
The rapid dissemination of information on social media has many positives but has also raised significant concerns regarding misinformation, making it crucial to understand the factors driving information-sharing behavior.
Juanita Zainudin   +3 more
doaj   +1 more source

Learning the Vector Coding of Egocentric Boundary Cells from Visual Data. [PDF]

open access: yesJ Neurosci, 2023
Lian Y   +4 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

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