Results 111 to 120 of about 5,294,886 (309)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

VFX librarianship: designing a global asset library for a visual effects studio

open access: yes, 2020
This article is an anecdotal outline of the author's work and experiences creating an asset library at a visual effects studio. It aims to start the conversation about librarianship and visual resource management’s role within film production ...
DeMichelis, Nichole
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

2015 State of the Visual Resources Association

open access: yes, 2020
During the 2015 Annual Business Meeting of the Visual Resources Association in Denver, Colorado, the president highlighted the accomplishments and challenges of the Association in a state of the association presentation.
Paul, Elaine
core  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Redesigning Visual Resources Facilities for 21st Century Challenges

open access: yes, 2020
Changes in our profession have been dramatic over the past ten years, not only in the services we deliver but also in the spaces where we work. In this paper, visual resources professionals from Smith College, the Massachusetts College of Art and Design (
Whiteside, Ann   +5 more
core  

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

open access: yesFEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová   +2 more
wiley   +1 more source

Special Bulletin #7: Disaster Planning for Visual Resources Collections

open access: yes, 2020
While there has already been much written on disaster planning, including guidelines for libraries and museums, personal safety, and there are far more sophisticated publications on film conservation, the purpose of this bulletin is to provide a concise ...
Hawkos, Editor: Lise J.
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Scope Drift? More of a Sharp Turn: Visual Resources Director One Day, Architecture Librarian the Next

open access: yes, 2020
When the position of Director of Visual Resources was transferred from the School of Architecture, Planning and Preservation (the School) to the University Libraries a dramatic scope drift occurred.
Frank, Cynthia G.
core  

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