Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Impact of Tinted Lenses on Contrast Sensitivity, Color Vision, and Visual Reaction Time in Young Adults. [PDF]
Seelan Samuel S +2 more
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
Effect of premenstrual tension on audiovisual reaction time
Background: Premenstrual tension has been known since long to affect the behavioural and physical well being of women. Behavioural differences in emotional and cognitive processing have been reported during premenstrual phase in women of reproductive age.
Vaishali M Paunikar, D Rajashree
doaj +1 more source
The Effect of Using Reactive Agility Exercises with the FITLIGHT Training System on the Speed of Visual Reaction Time and Dribbling Skill of Basketball Players. [PDF]
Hassan AK, Alhumaid MM, Hamad BE.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Effect of chewing tobacco on reaction time – A comparative study
Background: The smoked and smokeless forms of tobacco consist of about 4000 chemical constituents having carcinogenic potential and nicotine is the major among these.
Varsha M Shindhe +5 more
doaj +1 more source
Effect of helmet use on visual and auditory reaction time and peripheral field of vision
Background: In many Asian countries, motorcycles are an important means of transportation. The effectivity of helmets in reducing head injuries is studied, but the effect of helmets on the auditory and visual reaction time and peripheral field of vision ...
Prema Krishnarao Joshi +2 more
doaj +1 more source
Two separate, large cohorts reveal potential modifiers of age-associated variation in visual reaction time performance. [PDF]
Talboom JS +17 more
europepmc +1 more source

