Results 61 to 70 of about 234,956 (309)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Standalone and embedded stereo visual odometry based navigation solution [PDF]

, 2015
© Cranfield University, 2014This thesis investigates techniques and designs an autonomous visual stereo based navigation sensor to improve stereo visual odometry for purpose of navigation in unknown environments. In particular, autonomous navigation in a
Chermak, Lounis
core  

The visual object tracking VOT2016 challenge results

, 2016
777823The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published
Becker, Stefan, Krah, Sebastian, Arens, Michael, Matas, Jiri, Kristan, Matej, Leonardis, Ales, et al., Hübner, Wolsgang   +7 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The thermal infrared visual object tracking VOT-TIR2016 challenge results

, 2016
824849The Thermal Infrared Visual Object Tracking challenge 2016, VOT-TIR2016, aims at comparing short-term single-object visual trackers that work on thermal infrared (TIR) sequences and do not apply pre-learned models of object appearance.
Kristian, Matej, Becker, Stefan, Arens, Michael, Krah, Sebastian, Hübner, Wolfgang, Matas, Jiri, Felsberg, Michael, et al.   +7 more
core   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

VLFSE: Enhancing visual tracking through visual language fusion and state update evaluator

Machine Learning with Applications
Recently, visual tracking algorithms have achieved impressive results by combining dynamic templates. However, the instability of visual images and the incorrect timing of template updates lead to decreased tracking accuracy and stability in intricate ...
Fuchao Yang, Mingkai Jiang, Qiaohong Hao, Xiaolei Zhao, Qinghe Feng   +4 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

A Voronoi-Based Sensor Handover Protocol for Target Tracking in Distributed Visual Sensor Networks

International Journal of Distributed Sensor Networks, 2014
Target tracking is one of the important applications of wireless sensor networks. For a visual sensor network, the visual sensors have particular characteristics such as directional sensing, limited angle of view, and line-of-sight view.
Tien-Wen Sung, Chu-Sing Yang
doaj   +1 more source