Results 111 to 120 of about 4,310,555 (258)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Extended Operation of Stirling Convertors at NASA Glenn Research Center [PDF]

open access: yes, 2012
NASA Glenn Research Center (GRC) has been supporting development of free-piston Stirling conversion technology for spaceflight electrical power generation since 1999.
Oriti, Salvatore, M.
core  

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Design of supply source with several outputs

open access: yes, 2008
The main objective of this work is a design of supply power source with several outputs for a telephone subscriber module. This module consists of two parts.
Lačný, Radek
core  

Development of Electronic Load Controllers for Free-Piston Stirling Convertors Aided by Stirling Simulation Model [PDF]

open access: yes, 2004
The free-piston Stirling convertor end-to-end modeling effort at the NASA Glenn Research Center has produced a software-based test bed in which free-piston Stirling convertors can be simulated and evaluated.
Regan, Timothy F.
core  

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Extended Operation Testing of Stirling Convertors in Support of Stirling Radioisotope Power System Development [PDF]

open access: yes, 2008
100 We class Stirling convertors began extended operation testing at NASA Glenn Research Center (GRC) in 2003 with a pair of Technology Demonstration Convertors (TDCs) operating in air.
Wilson, Scott D.   +6 more
core  

Monolithically Integrated Polarization Mode Convertors with Semiconductor Lasers

open access: yes, 2014
Half and full polarization mode convertors which are monolithically integrated with semiconductor lasers are reported. A polarization mode conversion of above 40% and 88% was obtained using single and two back-to-back PMCs ...
Akbar, J.   +13 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

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