Quantitative RNA modification mapping by mass spectrometry with isobaric tags and nucleobase fragment analysis. [PDF]
Wu J +7 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Accurate and Sensitive Multiplexed Proteomics Using the 7-Plex DiLeuC Complementary Ion Strategy. [PDF]
Zhang J, Wang Z, Lu Y, Yao Y, Li L.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Single-Phase Voltage-Doubler Rectifier with Balanced Capacitor Voltages
Kiyotake, Hirofumi +3 more
openaire +2 more sources
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
A light and modular glovebox for hazardous material machining. [PDF]
Negri SP, Nitti M, Mosca N, Renò V.
europepmc +1 more source
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez +4 more
wiley +1 more source
Cardiac arrhythmia detection via PQRST analyzed data using an optimized hierarchical fused fuzzy deep reinforcement learning. [PDF]
Mahdavi N +6 more
europepmc +1 more source
G3'MTMD3 in the insect GABA receptor subunit, RDL, confers resistance to broflanilide and fluralaner. [PDF]
Zhang Y +10 more
europepmc +1 more source

