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Monoclonal antibody development and antigenic epitope identification of infectious bursal disease virus VP5

The Veterinary Journal
Infectious bursal disease (IBD) is an important immunosuppressive disease affecting chickens and is caused by infectious bursal disease virus (IBDV) infection. VP5 is a non-essential protein for IBDV replication but plays a critical role in IBDV pathogenesis. A deeper understanding of the biological functions of VP5 is lacking.
Jinze, Han   +20 more
openaire   +2 more sources

Molecular cloning and prokaryotic expression of vp5 gene of grass carp reovirus strain GCRV096

Virus Genes, 2013
VP5 is an outer capsid protein of grass carp reovirus (GCRV). It is predicted to involve in helping GCRV enter the host cells. In this study, the full-length vp5 gene (accession number in GenBank: JN206664.1) was cloned from GCRV strain GCRV096, which was isolated from diseased grass carp (Ctenopharyngodon idella) in southern China by RT-PCR technique ...
Ji-chang, Jian   +5 more
openaire   +2 more sources

Identification of three novel linear B-cell epitopes on the VP5 protein of BTV16

Veterinary Microbiology, 2013
Bluetongue virus (BTV) VP5 protein is an important antigenic protein which is centrally involved in serotype determination and the virus entry process. Very little is known about the B-cell epitopes on the BTV VP5 protein recognized by humoral immune responses.
Wen-Shi, Wang   +11 more
openaire   +2 more sources

Expression of the outer capsid proteins VP2 and VP5 of bluetongue virus in Saccharomyces cereuisiae

Virus Research, 1994
cDNAs transcribed from bluetongue virus serotype 1 (Australia) ds RNA 2 and ds RNA 6 coding for the major neutralising antigen VP2 and the outer capsid protein VP5, respectively, were amplified in polymerase chain reactions and ligated downstream of the copper-inducible metallothionein promoter in the yeast expression plasmid pYELC5.
J C, Martyn, A R, Gould, M, Yu
openaire   +2 more sources

TRIM103 activates the RLRs pathway to enhance antiviral response by targeting VP5 and VP7

Developmental & Comparative Immunology
Grass carp (Ctenopharyngodon idella), crucial to global inland aquaculture with a production of 5.8 million tones in 2020, faces significant challenges from hemorrhagic disease caused by grass carp reovirus (GCRV). Rapid mutations compromise current vaccines, underscoring the need for a deeper understanding of antiviral mechanisms to enhance molecular ...
Beibei Qin   +4 more
openaire   +2 more sources

[Cloning of infectious bursal disease virus (Gt strain) lack of VP5 gene].

Wei sheng wu xue bao = Acta microbiologica Sinica, 2009
Infectious bursal disease virus (IBDV) belongs to genus Avibirnavirus of the family Birnaviridae. The genome of IBDV consists of two segments of double-strand RNA, which encode four structural protein VP1-VP4 and one non-structural protein VP5.To study the function of VP5 of IBDV, the recombinant virus, lack of VP5 gene, was constructed and rescused by
Liting, Qin   +5 more
openaire   +1 more source

Characteristics and functional roles of VP5 protein of herpesviruses

Reviews in Medical Microbiology, 2013
Bihong Dai, Anchun Cheng, Mingshu Wang
openaire   +1 more source

VP5-13 Topology Optimization for a Magnetic Actuator Using Different Gradient-Based Solvers

This paper presents a comparative study on density based topology optimization for a magnetic actuator using different gradient-based solvers. The applied solvers are method of moving asymptotes (MMA), interior point optimizer (IPOPT), and sparse nonlinear optimizer (SNOPT).
Mohamed Reda Mahmoud   +2 more
openaire   +1 more source

Gene Cloning and Sequence Analysis of VP5 for Chicken Infectious Bursal Disease Virus

2010
Chicken infectious bursal disease viruses (IBDV) were isolated and purified from infected bursa and then the viruses were used to extract genomic RNA.The cDNA first strand was synthesized by reverse transcription using IBDV genomic RNA as the template.A DNA fragment coding for the viral protein 5(VP5) of IBDV was amplified by PCR,which was ligated with
Leung, FCC, Liu, CR, Yu, SQ
openaire   +2 more sources

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