Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Chlorhexidine as the optimal disinfection method for an accidentally dropped autograft during an anterior cruciate ligament reconstruction surgery: A double-blind study. [PDF]
Pourheidar S, Moayedfar M, Jabalameli M.
europepmc +1 more source
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source
Application of image analysis combined with regression models to estimate the reduction of Escherichia coli and Salmonella spp. on vegetable surfaces after washing. [PDF]
Chengsa-Ard F +10 more
europepmc +1 more source
Excess Ca2+ ions activate the Calcium‐Sensing Receptor (CaSR), which subsequently drives the uptake of excess inorganic phosphate (Pi) via the Pi transporter (Pit−1) in chondrocytes. This mechanism causes a toxic increase in intracellular Pi concentration, ultimately leading to chondrocyte apoptosis and pathological mineralization. Excess extracellular
Sachie Nakatani +7 more
wiley +1 more source
Impact of ultrasound-assisted single washing process on tilapia mince: Biochemical changes, structural modifications, and gel formation. [PDF]
Hamzeh A +4 more
europepmc +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
The Influence of PVDF Membrane Ageing on the Efficiency of Bacterial Rejection During the Ultrafiltration Treatment of Carwash Wastewater. [PDF]
Woźniak P, Gryta M.
europepmc +1 more source

