Results 21 to 30 of about 101,455 (263)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The Fusion of Infrared and Visible Images via Feature Extraction and Subwindow Variance Filtering

Journal of Electrical and Computer Engineering
This paper presents a subwindow variance filtering algorithm for fusing infrared and visible light images, with the goal of addressing challenges related to blurred details, low contrast, and missing edge features. First, images to be fused are subjected
Xin Feng, Haifeng Gong
doaj   +1 more source

A bibliometric analysis of research on manganese sand (1993–2024): Insights from the web of science core collection

Next Materials
Manganese sand is a cost-effective and efficient material widely used in water treatment. This work presents a comprehensive bibliometric analysis of research trends on manganese sand from 1993 to 2024, based on data from the Web of Science Core ...
Bo Song, Zhenxing Shen, Zitong Wang, Qin Peng, Xinyu Mo, GuiLing Zhou, Jia Liu   +6 more
doaj   +1 more source

Hot Deformation Behavior and Processing Map of High-Strength Nickel Brass

Metals, 2020
The flow behavior of a new kind of high-strength nickel brass used as automobile synchronizer rings was investigated by hot compression tests with a Gleeble-3500 isothermal simulator at strain rates ranging from 0.01 to 10 s−1 and a wide deformation ...
Qiang Liang, Xin Liu, Ping Li, Xianming Zhang   +3 more
doaj   +1 more source

Super‐Refractory Status Epilepticus (SRSE) in a Patient With Compound Heterozygous OPA1 Variants: Case Report and Literature Review

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Super‐Refractory Status Epilepticus (SRSE) is a rare, life‐threatening neurological emergency with unclear etiology in many cases. Mitochondrial dysfunction, often due to disease‐causing genetic variants, is increasingly recognized as a cause, with each gene producing distinct pathophysiological mechanisms.
Pouria Mohammadi, Lara Basovic, Christopher Michael McGraw   +2 more
wiley   +1 more source

Single‐Atom Cobalt Species on Curved Hollow Carbon Sphere: Breaking Linear Scaling Relationship Limitations in Electrocatalytic Deactivation of Dilute Antibiotic Pollutants

Advanced Science
Despite maximal atomic efficiency, single‐atom catalysts (SACs) are constrained by linear scaling relationships in electrocatalytic hydrodechlorination (ECHD), a process effective for antibiotic pollutant deactivation yet challenged by multi‐proton ...
Jiahong Zou, Shenbin Zheng, Chunyang Dan, Huimin Sui, Wenyang Fu, Xiaoshu Lv, Boping Ren, Guangming Jiang, Hong Liu   +8 more
doaj   +1 more source

GAD65 Antibody ELISA With Extended Reportable Range: Validation and Guidance for Neurological Practice

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon, Dana Olofson, Diana Anissian, Divyanshu Dubey, Eoin P. Flanagan, Max C. Herman, Sarosh R. Irani, Daniel H. Lachance, Maria A. Willrich, Sean Pittock, John R. Mills, Anastasia Zekeridou   +11 more
wiley   +1 more source

Choroid Plexus Enlargement and USPIO‐Based Inflammatory Feature in Cerebral Small Vessel Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The choroid plexus (CP) is a key component of the blood–cerebrospinal fluid barrier (BCSFB), but its mechanism of action in cerebral small vessel disease (CSVD) remains unclear. This study investigated CP volume (CPV) alterations and their association with conventional imaging markers in CSVD and explored the underlying role of ...
Yongqiang Qu, Xiaoning Ren, Cuihua Fan, Chun Li, Lijie Zhang, Zaiqiang Zhang, Shaowu Li, Zhongmeng Lai, Bin Cai, Ying Fu, Yi Lin, Zhibao Zhu   +11 more
wiley   +1 more source

Compost and Vermicompost as Substrates Enriched with Trichoderma asperellum for the Production of Basic Potato Seed in the Venezuelan Andes

Horticulturae
The agricultural sector faces significant pressure to meet the growing global demand for food while managing the planet’s limited natural resources. Addressing this challenge requires the strategic use of abundant materials and accessible biotechnologies
Haydee Peña, Fernando Diánez, Beatriz Ramírez, José Sulbarán, Karen Arias, Victoria Huertas, Mila Santos   +6 more
doaj   +1 more source