Results 41 to 50 of about 1,020,270 (277)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

FORMATION OF NANOCRYSTAL STATE IN DETAILS OF CARBON STRUCURAL STEELS

Литьë и металлургия, 2018
The conditions of formation of the nanocrystal state of the structure in carbon steels are considered in the article. The methods of research and experimental data on the microstructural structure of the strengthened parts of the working bodies of ...
A. A. Andrushevich, G. I. Aniskovich, p L. Kantor, D. V. Kuchuk   +3 more
doaj   +1 more source

Piped water cooling of concrete dams [PDF]

, 2004
Piped water is used to remove hydration heat from concrete dams during construction. By examining simple models we obtain an estimate for the temperature rise along the pipe network and within the concrete.
Ballim, Y., Charpin, J., Fitt, A.D., Fowkes, N., Myers, T., Patini, A.P.   +5 more
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Traditional Biocidal Replacement Viability of Microcrystalline Silver Chloride [PDF]

, 2017
The antimicrobial effects of silver ions and silver chloride nanoparticles have been well established while the efficacy of microcrystalline silver chloride has been less studied.
Curtis, R., Hemminger, Zachary, Picardo, Kristin F., Walsh, P.   +3 more
core   +1 more source

Cooling jug physics

, 2017
We discuss the physics of the pot-in-pot cooler. By balancing temperature decrease due to evaporation and temperature increase due to heat exchange, we find the equilibrium temperature of the pot.
Golubov, Oleksiy, Luniachek, Oleh, Timchenko, Ruslan   +2 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Meeting Global Cooling Demand with Photovoltaics during the 21st Century

, 2009
Space conditioning, and cooling in particular, is a key factor in human productivity and well-being across the globe. During the 21st century, global cooling demand is expected to grow significantly due to the increase in wealth and population in sunny ...
Buonassisi, Tonio, Laine, Hannu S., Looney, Erin E., Peters, Ian Marius, Salpakari, Jyri, Savin, Hele   +5 more
core   +2 more sources

Structural Order in Glassy Water

, 2005
We investigate structural order in glassy water by performing classical molecular dynamics simulations using the extended simple point charge (SPC/E) model of water.
C. Kittel, Francesco Sciortino, H. Eugene Stanley, Nicolas Giovambattista, O. Mishima, P. G. Debenedetti, Pablo G. Debenedetti, R. Zallen, S. Torquato   +8 more
core   +2 more sources