Results 91 to 100 of about 722,593 (299)
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Rehabilitating The Artificial Ponds With Geosynthetic Clay Liners - A Case Study [PDF]
, 2013 Geosynthetic Clay Liners (GCLs) offer engineers, owners and contractors safe and mostly economic solutions to everyday engineering challenges encountered particularly in hydraulic engineering applications and especially in the liquid and waste landfill ...
Eyyub Karakan +2 more
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Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Energy Savings and Sustainability Opportunities at US Army Corps of Engineers Facilities: A Guide to Identify, Prioritize, and Estimate Projects at Complexes That Have Not Conducted a Facility-Level Energy and Water Evaluation [PDF]
, 2012 Abstract : Executive Order (EO) 13514, Federal Leadership in Environmental, Energy, and Economic Performance, expanded on the energy re-duction and environmental performance requirements of Executive Order 13423, Strengthening Federal Environmental, Energy, and Transportation Management, by adding sustainability requirements and performance planning ...
Laura Curvey, David M. Underwood
openaire +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Carbon Capture Clustering: the Case for Coordinated Approaches to Address Freshwater Use Concerns [PDF]
, 2015 Carbon capture and storage (CCS) will be a key technology for reducing emissions from fossil-fuelled electricity generation. The UK is developing demonstration plants and UK Government strategy proposes the clustering of CCS facilities, having ...
Amezaga, J.M., Byers, E.A., Hall, J.W.
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Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Community Response Strategies for Environmental Problems of Water Supply and Wastewater Disposal in Fairbanks, Alaska [PDF]
, 1975 This report examines the history of the response strategies of the Fairbanks, Alaska, community to problems of water supply and wastewater disposal.
Pearson, Roger W., Smith, Daniel W.
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