Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Reasons for Resorting to Organic Farming and Advantages Perceived by the Organic Farmers [PDF]
The study mainly focused on reasons for resorting to organic farming and advantages and benefits perceived by the organic farmers. The study was conducted in Coimbatore, Erode and Dharampuri districts of Tamil Nadu.
Elavarasi, M., Ponnusamy, K.A.
core +2 more sources
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Regression-derived optimal milk thistle in growing quail's diet
: A study was conducted to evaluate the nutritional benefits of milk thistle (Silybum marianum) in quail nutrition as an additive containing antioxidant compounds such as silymarin. A total of 300, 14-d old Japanese quail chicks were randomly allotted to
Mahmoud Ghazaghi +3 more
doaj +1 more source
Perubahan Sifat Fisik Gambut Akibat Kebakaran di Desa Teluk Binjai Kecamatan Teluk Meranti Kabupaten Pelalawan [PDF]
This research is conducted to compare the physical characteristic change between burnt & unburnt peat soil and also to understand its change on both seven days & four months after burn.
Amri, A. I. (Al) +2 more
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Effects of Different High Hydrostatic Pressure Treatments on Gel Formation of Pea 7S and 11S Globulins [PDF]
In this study, the rheological and textural properties and water-holding capacity of pea 7S and 11S globulin gels induced by high hydrostatic pressure (HHP) at 100–500 MPa for 10 min were measured.
WANG Xueyan, LI Kaixin, LI Jiahao, MA Lingjun, CHEN Fang, HU Xiaosong, JI Junfu
doaj +1 more source
Modifikasi Protein Dedak Jagung dengan Gum Xanthan untuk Pengembangan Sifat Fungsional Menggunakan Ion Kalsium [PDF]
Interaksi protein dedak jagung dan gum xanthan bertujuan untuk memperbaiki sifat fungsional sehingga dapat diaplikasikan dalam berbagai produk pangan.Metode penelitian meliputi tahapan pencucian lemak, pemisahan isolat dengan teknik pelarutan pada pH 8,5
Sudiyono, S. (Sudiyono) +1 more
core
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley +1 more source
Pengaruh Perbedaan Konsentrasi Karagenan Terhadap Karakteristik Empek- Empek Udang Windu (Penaeus Monodon) [PDF]
Udang merupakan hasil perikanan yang mengandung protein tinggi, daging udang dapat diolah sebagai makanan olahan seperti empek-empek udang dengan kandungan protein tinggi. Empek-empek udang dapat menjadi produk olahan diversifikasi yang diminati.
Ma\u27ruf, W. F. (Widodo) +2 more
core

