Results 31 to 40 of about 1,484,040 (218)

Effect of curing and storage temperature on shelf life of onion (Allium cepa L.) bulbs

Fundamental and Applied Agriculture, 2020
Simple and low cost farm technologies are required for small-scale farmers in developing countries to improve marketability and reduce postharvest losses of onion (Allium cepa L.) bulbs. Two experiments were conducted to evaluate the effect of curing and
Assinapol Ndereyimana, Shinichi Koyama, Boniface Kagiraneza   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Variation in stem bark conductance to water vapor in Neotropical plant species

Frontiers in Forests and Global Change
Bark conductance to water vapor (gbark) is an important determinant of drought tolerance in tropical plants. Examining species differences in bark conductance can provide useful information about the resilience of tropical trees to ongoing climate change.
Eleinis Ávila-Lovera, Klaus Winter
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Características da chuva e perdas por erosão sob diferentes práticas de manejo do solo Rainfall characteristics and erosion losses for different soil management practices

Revista Brasileira de Engenharia Agrícola e Ambiental, 2009
Objetivou-se com este trabalho, avaliar as relações entre a erosividade da chuva e os padrões da precipitação com as perdas por erosão, para diferentes tipos de preparo do solo em um Argissolo Vermelho-Amarelo.
Daniel F. de Carvalho, Eleandro S. da Cruz, Marinaldo F. Pinto, Leonardo D. B. Silva, José G. M. Guerra   +4 more
doaj   +1 more source

Use of hydraulic model in real water loss reduction and water distribution network operational cost lowering

Economics and Environment, 2022
Most of the small water companies supplying a small number of consumers with water are struggling with the extremely tight budget, often making any large-scale modernisation impossible.
Artur Zajkowski, Łukasz Wysocki, Piotr Tuz, Izabela Bartkowska, Wojciech Kruszynski   +4 more
doaj   +1 more source