Results 141 to 150 of about 8,064,225 (347)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Measurement of radon concentrations at Super-Kamiokande

, 1999
Radioactivity from radon is a major background for observing solar neutrinos at Super-Kamiokande. In this paper, we describe the measurement of radon concentrations at Super-Kamiokande, the method of radon reduction, and the radon monitoring system.
A Habig, A Hasegawa, A Kibayashi, A Okada, A Sakai, A Suzuki, A.L Stachyra, A.T Suzuki, B Viren, B.K Kim, C Mauger, C McGrew, C Saji, C Yanagisawa, C.K Jung, C.W Walter, D Casper, D Kielczewska, D Takemori, E Blaufuss, E Kearns, E Sharkey, F Tsushima, Fukuda, G.W Sullivan, H Fujiyasu, H Ishino, H Ogawa, H Okazawa, H Takeuchi, H.W Sobel, J Hill, J Kameda, J Kanzaki, J Shirai, J.A Goodman, J.G Learned, J.L Stone, J.S George, K Higuchi, K Inoue, K Ishihara, K Kaneyuki, K Kobayashi, K Martens, K Miyano, K Nakamura, K Nishijima, K Nishikawa, K Okumura, K Scholberg, K.K Young, K.S Ganezer, L.L Wai, L.R Price, L.R Sulak, M Earl, M Etoh, M Goldhaber, M Kirisawa, M Koga, M Kohama, M Koike, M Koshiba, M Miura, M Nakahata, M Nemoto, M Sakuda, M Shiozawa, M Smy, M Takahashi, M Takahata, M Takita, M Yoshida, M.D Messier, M.L Chen, M.R Vagins, N Sakurai, Nemoto, O Sasaki, R Sanford, R Svoboda, R.J Wilkes, R.W Ellsworth, S Echigo, S Hatakeyama, S Inaba, S Kasuga, S Matsuno, S Mine, S Nakayama, S Tasaka, S Yamada, S.B Kim, T Barszczak, T Futagami, T Hasegawa, T Hayakawa, T Ishii, T Iwamoto, T Kajita, T Kobayashi, T Maruyama, T Yamaguchi, T.J Haines, Takeuchi, Tasaka, V.J Stenger, W Doki, W Gajewski, W.E Keig, W.R Kropp, Y Fukuda, Y Hatakeyama, Y Hayato, Y Itow, Y Kanaya, Y Kobayashi, Y Koshio, Y Nagashima, Y Obayashi, Y Oyama, Y Suzuki, Y Takeuchi, Y Totsuka, Y Watanabe   +125 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Application of Motionless Mixer in Gas Purification – A case study [PDF]

, 2001
The lecture gives a review on the application of motionless mixers in gas purification, where wet dust separation is carried out by water circulation trough vertical tubes filled with FixMix(TM) motionless mixer elements.
Bucsky, Gy., Gyenis, János, Ujhidy, Aurél   +2 more
core  

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Evaluation of bacteriological and chemical quality of dialysis water and fluid in Isfahan, central Iran [PDF]

, 2016
Background: Chemical and microbial quality of water used in hemodialysis play key roles in a number of dialysisrelated complications. In order to avoid the complications and to guarantee safety and health of patients therefore, vigorous control of water ...
Hassanzadeh, A., Hatamzadeh, M., Nikaeen, M., Shahryari, A., Vahid Dastjerdi, M.   +4 more
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Smart and innovative nanotechnology applications for water purification

Hybrid Advances, 2023
Nishu, Sudesh Kumar
semanticscholar   +1 more source