SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Drainage lysimeter based measurement of water requirement and crop coefficient of bread wheat under semi-arid climate of Melkassa, Ethiopia. [PDF]
Bayisa GD +4 more
europepmc +1 more source
Actual crop water use in project countries : a synthesis at the regional level [PDF]
This report aims to synthesize the results of a crop water use study conducted by country teams of the GEF/World Bank project, Regional Climate, Water, and Agriculture: Impacts on and Adaptation of Agro-ecological Systems in Africa.
Maraux, Florent +2 more
core
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
Retraction: Application of edge computing and GIS in ecological water requirement prediction and optimal allocation of water resources in irrigation area. [PDF]
PLOS ONE Editors.
europepmc +1 more source
KHS‐Cnd peptide is able to impair biofilm formation and disaggregate mature biofilms in Acinetobacter baumannii clinical isolates. Differences in extracellular metabolites reflect changes in biofilm metabolism due to KHS‐Cnd treatment. Among the differentially represented extracellular metabolites upon KHS‐Cnd treatment, the significantly altered ...
Fernando Porcelli +9 more
wiley +1 more source
Crop water requirement and irrigation scheduling under climate change scenario, and optimal cropland allocation in lower kulfo catchment. [PDF]
Reta BG, Hatiye SD, Finsa MM.
europepmc +1 more source
ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori +8 more
wiley +1 more source
Assessing the impact of climate change on water requirement and yield of sugarcane over different agro-climatic zones of Tamil Nadu. [PDF]
Guhan V +6 more
europepmc +1 more source
TMC4 localizes to multiple taste cell types in the mouse taste papillae
Transmembrane channel‐like 4 (TMC4), a voltage‐dependent chloride channel, plays a critical role in amiloride‐insensitive salty taste transduction. TMC4 is broadly expressed in all mature taste cell types, suggesting a possible involvement of multiple cell types in this pathway.
Momo Murata +6 more
wiley +1 more source

