Results 51 to 60 of about 2,158,364 (252)

Cold storage as a method for the long-term preservation of tropical dissolved organic carbon (DOC) [PDF]

open access: yesMires and Peat, 2016
Fluvial fluxes of dissolved organic carbon (DOC) may represent an important loss for terrestrial carbon stores in the tropics. However, there is currently limited guidance on the preservation of tropical water samples for DOC analysis.
S. Cook   +6 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

open access: yesFEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

open access: yesFEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa   +3 more
wiley   +1 more source

Fe3O4/GO nanocomposite modified glassy carbon electrode as a novel voltammetric sensor for determination of bisphenol A

open access: yesJournal of Electrochemical Science and Engineering, 2022
A new voltammetric sensor is proposed for the determination of bisphenol A, using a glassy carbon electrode (GCE) modified with Fe3O4/graphene oxide (GO) nanocomposite.
Fariba Beigmoradi, Hadi Beitollahi
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

open access: yesFEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto   +3 more
wiley   +1 more source

Determination of Lead, Cadmium, Copper and Iron Contents in Water by Multi-walled Carbon Nanotubes-solid Phase Extraction

open access: yesYankuang ceshi, 2014
The traditional solid phase extraction sorbent contains certain deficiencies in the progress of processing heavy metals in environmental samples, such as pH instability and co-extraction difficulty.
SUN Na   +3 more
doaj  

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

open access: yesFEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas   +6 more
wiley   +1 more source

Efficient Hydrothermal Synthesis of Flake-Like Molybdenum Disulfide for Selective Electrochemical Detection of Metol in Water Real Samples

open access: yesInternational Journal of Electrochemical Science, 2020
Transition metal chalcogenide has great interest owing to unique structural properties, higher conductivity, larger electroactive surface area, and excellent catalytic response.
Chelliah Koventhan   +6 more
doaj   +1 more source

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