Results 61 to 70 of about 468,655 (259)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Comparison of CGMS-WOFOST and HYDRUS-1D Simulation Results for One Cell of CGMS-GRID50

Soil and Water Research, 2006
CGMS (Crop Growth Monitoring System) developed by JRC is an integrated system to monitor crop behaviour and quantitative crop yield forecast that operates on a European scale.
Radka Kodešová, Lukáš Brodský
doaj   +1 more source

ON THE WATER SURFACE PROFILE AROUND BRIDGE PIER

Transactions of the Japan Society of Civil Engineers, 1959
In this paper, the author introduced a fundamental equation for water flow with vertical velocity distribution taking the flow bed into consideration. This equation is isommorphie with the fundamental equation of the subsonic flow of air. And the author performed calculation on the water surface profile around bridge pier by applying the Imai's theory ...
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

PROFIL MUKA AIR DI HULU GROUNDSILL TIPE AMBANG LEBAR DAN OGEE

Dinamika Rekayasa, 2007
A Groundsill is built with main purpose to control river bed. When groundsill is placed in a river there will cause water surface raises and creates backwater in upstream of the groundsill. It influences elevation of flood and inundation.
Wahyu Widiyanto
doaj  

Characterization of Drain Surface Water: Environmental Profile, Degradation Level and Geo-statistic Monitoring [PDF]

Pakistan Journal of Analytical & Environmental Chemistry, 2015
The physico-chemical characterization of the surface water. Samples was carried out collected from nine sampling points of drain passing by the territory of Hafizabad city, Punjab, Pakistan.
Muhammad Waseem Mumtaz, Muhammad Asam Raza, Syed Makhdoom Hussain, Muhammad Imran, Muhammad Nadeem Abbas, Zohaib Ahmed, Majid Hussain   +6 more
doaj  

Effect of process parameters on depth of penetration and topography of AZ91 magnesium alloy in abrasive water jet cutting

Journal of Magnesium and Alloys, 2018
In the present study, the influence of dynamic process parameters such as water pressure, traverse speed and abrasive mass flow rate on depth of penetration and surface topography in high strength AZ91 magnesium alloy were investigated using Abrasive ...
C A Niranjan, S Srinivas, M Ramachandra
doaj   +1 more source