Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Identification of time points for LNG throughput: a complex network approach
Critical nodes play a decisive role in shaping the intrinsic structure of complex networks. They help uncover the interconnections within real-world systems. Recognizing these key nodes is a central topic in complex network research.
Bohao Liu +4 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Sediment dynamics in the Bohai Sea under extreme weather: advances and prospects
Extreme weather events are major drivers of coastal geomorphic change,and understanding the mechanisms governing sediment-dynamic responses to these events is important for resilience-oriented coastal management.
Zhangyi ZHAO +6 more
doaj +1 more source
Wave Analysis for West Coast of South Myanmar
The characteristic of southern parts of Myanmar is tropical monsoon climate, and this area is affected by few typhoons. The wave height is changed with season, the field measured data shows that the aver-age monthly maximum wave height is in June.
Xu Yanan +3 more
doaj +1 more source
Research on the driving characteristics of motor vehicles in high-altitude areas for the analysis of exhaust emission characteristics [PDF]
Research on the driving characteristics of motor vehicles at different altitudes in plateau regions and road emission intensity can provide technical support for energy conservation and emission reduction policies in plateau areas.
Qi Zhaoyu +5 more
doaj +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Methodological Study on Voluntary Greenhouse Gases Reduction for Shore Power System [PDF]
Voluntary emission reduction of greenhouse gases is conducive to reducing carbon dioxide (CO2) emissions and fostering a carbon trading market. Voluntary greenhouse gas emission reduction methodologies can be used to determine project baselines ...
Liu Leilei +3 more
doaj +1 more source
Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source

