Results 11 to 20 of about 53 (46)
Engineering plant virus resistance: from RNA silencing to genome editing strategies
Summary Viral diseases severely affect crop yield and quality, thereby threatening global food security. Genetic improvement of plant virus resistance is essential for sustainable agriculture. In the last decades, several modern technologies were applied in plant antiviral engineering.
Yaling Zhao +3 more
wiley +1 more source
SPREAD OF WHEAT DWARF VIRUS (WDV) ON THE SOUTH OF UKRAINE
На посівах пшениці і ячменю південного регіону України виявлено вірус карликовості пшениці (ВКП). Вірус ідентифіковано за допомогою імуноферментного аналізу (IФА) і полімеразної ланцюгової реакції (ПЛР). Проаналізовано поширення вірусу, вивчено сортову чутливість злакових культур до ВКП.
openaire +3 more sources
Single Cell Mechanics in Disease Progression
Cells transmit distinct mechanical forces through ECM adhesion and cell–cell junctions and actomyosin‐generated traction forces are transmitted to the substrate through integrin‐based focal adhesions. Mechanical signals are further transmitted to the nucleus via the LINC complex, connecting the cytoskeleton to the nuclear lamina. These forces integrate
Sabin Kim +3 more
wiley +1 more source
Summary Cucumber mosaic virus (CMV) is a highly prevalent viral pathogen causing substantial damage to the bulb and cut‐flower production of Lilium spp. Here, we performed an Illumina RNA sequencing (RNA‐Seq) study on the leaf tissues of a virus‐resistant species Lilium regale inoculated with mock control and CMV.
Daoyang Sun +7 more
wiley +1 more source
Elucidating R‐gene‐mediated resistance to wheat dwarf virus in Triticum aestivum genotypes
This study investigates R‐gene‐mediated resistance to wheat dwarf virus (WDV) among different wheat genotypes. Following inoculation with Psammotettix alienus (leafhoppers) and the subsequent appearance of characteristic symptoms such as yellowing and dwarfing, phenotypic responses were evaluated alongside molecular analyses, including quantification ...
Priyanka Krishnamurthy +3 more
wiley +1 more source
Summary Plant RNA virus‐based guide RNA (gRNA) delivery has substantial advantages compared to that of the conventional constitutive promoter‐driven expression due to the rapid and robust amplification of gRNAs during virus replication and movement. To date, virus‐induced genome editing tools have not been developed for wheat and maize.
Jiacheng Hu +9 more
wiley +1 more source
Wheat Dwarf Virus as a Modulator of Multi‐Stress Responses in Wheat
ABSTRACT Wheat dwarf virus (WDV) is an emerging constraint to cereal production whose epidemiological significance has intensified under climate change. Rising temperatures, extended vector activity, and the expansion of Psammotettix alienus into new regions have increased both the frequency and severity of WDV outbreaks.
Jana Asszonyi
wiley +1 more source
Cas9‐Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR‐mediated genome editing
Summary Genome editing based on the homology‐directed repair (HDR) pathway enables scar‐free and precise genetic manipulations. However, the low frequency of HDR hinders its application in plant genome editing. In this study, we engineered the fusion of Cas9 and a viral replication protein (Rep) as a molecular bridge to tether donor DNA in vivo, which ...
Zhentao Zhou +7 more
wiley +1 more source
Beyond a few bases: methods for large DNA insertion and gene targeting in plants
SUMMARY Genome editing technologies like CRISPR/Cas have greatly accelerated the pace of both fundamental research and translational applications in agriculture. However, many plant biologists are functionally limited to creating small, targeted DNA changes or large, random DNA insertions.
Katie Vollen +2 more
wiley +1 more source
New genomic techniques can contribute to reduced pesticide usage in Europe
Can modern breeding technologies, such as genome editing, contribute to reduced pesticide usage? This question has been accentuated by a recent legal proposal to exempt genome‐edited plants from the strict regulations applied to classical genetically modified (GM) crops within the European Union (EU).
Jens F. Sundström +4 more
wiley +1 more source

