Results 161 to 170 of about 1,171,281 (357)

Experimental workflow from A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer

, 2023
Matthew P. Humphries, Sreekumar Sundara Rajan, Alastair Droop, Charlotte A.B. Suleman, Carmine Carbone, Cecilia Nilsson, Hedieh Honarpisheh, Gábor Cserni, Jo Dent, Laura Fulford, Lee B. Jordan, J. Louise Jones, Rani Kanthan, Maria Litwiniuk, Anna Di Benedetto, Marcella Mottolese, Elena Provenzano, Sami Shousha, Mark Stephens, Rosemary A. Walker, Janina Kulka, Ian O. Ellis, Margaret Jeffery, Helene Thygesen, Vera Cappelletti, Maria Grazia Daidone, Ingrid Hedenfalk, Marie-Louise Fjällskog, Davide Melisi, Lucy F. Stead, Abeer M. Shaaban, Valerie Speirs   +31 more
openalex   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Evo‐Scope: Fully automated assessment of correlated evolution on phylogenetic trees

Methods in Ecology and Evolution
Correlated evolution describes how multiple biological traits evolve together. Recently developed methods provide increasingly detailed results of correlated evolution, sometimes at elevated computational costs.
Maxime Godfroid, Charles Coluzzi, Amaury Lambert, Philippe Glaser, Eduardo P. C. Rocha, Guillaume Achaz   +5 more
doaj   +1 more source

Proof-of-concept engineering workflow demonstrator

, 2006
When Microsoft needed a proof-of-concept implementation of bespoke engineering workflow software for their customer, BAE Systems, it called on the software engineering skills and experience of the Microsoft Institute for High Performance Computing.
Cox, SJ, Molinari, M., Takeda, K
core  

Standardised workflow for mass spectrometry-based single-cell proteomics data processing and analysis using the scp package [PDF]

, 2023
Samuel Grégoire, Christophe Vanderaa, Sébastien Pyr dit Ruys, Gabriel Mazzucchelli, Christopher Kune, Didier Vertommen, Laurent Gatto   +6 more
openalex   +1 more source

Snakemake - a scalable bioinformatics workflow engine

Bioinform., 2018
Johannes Köster, S. Rahmann
semanticscholar   +1 more source