Results 131 to 140 of about 574,123 (330)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

On the use of an IMS LD ontology for creating and executing Units of Learning: Application to the Astronomy case study

Journal of Interactive Media in Education, 2008
This paper describes how the Astronomy case study is modelled, created and executed, using a IMS LD ontology. The ontology was aimed at overcoming the expressiveness limitations of the IMS LD XML Schema by means of a taxonomy of concepts and a set of ...
Eduardo Sánchez, Manuel Lama, Ricardo R. Amorim, Juan Carlos Vidal, Adrián Novegil   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

DIANA-microT web server v5.0: service integration into miRNA functional analysis workflows

Nucleic Acids Res., 2013
MicroRNAs (miRNAs) are small endogenous RNA molecules that regulate gene expression through mRNA degradation and/or translation repression, affecting many biological processes.
M. Paraskevopoulou, G. Georgakilas, Nikos Kostoulas, I. Vlachos, Thanasis Vergoulis, M. Reczko, Christos Filippidis, Theodore Dalamagas, A. Hatzigeorgiou   +8 more
semanticscholar   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Advancing Newborn Screening Long-Term Follow-Up: Integration of Epic-Based Registries, Dashboards, and Efficient Workflows

International Journal of Neonatal Screening
The Connecticut Newborn Screening (NBS) Network, in partnership with the Connecticut Department of Public Health, strategically utilized the Epic electronic health record (EHR) system to establish registries for tracking long-term follow-up (LTFU) of NBS
Katherine Raboin, Debra Ellis, Ginger Nichols, Marcia Hughes, Michael Brimacombe, Karen Rubin   +5 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Editorial: Data and Workflows for Multilingual Digital Humanities

Journal of Open Humanities Data
This Editorial presents the Special Collection ‘Data and Workflows for Multilingual Digital Humanities’. The Special Collection brings together an assortment of contributions that explore how power structures operate in digital knowledge production with ...
Lorella Viola
doaj   +1 more source

Semantic Description, Publication and Discovery of Workflows in myGrid

, 2004
The bioinformatics scientific process relies on in silico experiments, which are experiments executed in full in a computational environment. Scientists wish to encode the designs of these experiments as workflows because they provide minimal ...
Goble, Carole, Lord, Phillip, Miles, Simon, Moreau, Luc, Papay, Juri, Wroe, Chris   +5 more
core   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source