We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Efficacy of electronic apex locators in comparison with intraoral radiographs in working length determination- a systematic review and meta-analysis. [PDF]
Kaur G +10 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Postoperative pain evaluation in patients undergoing endodontic treatment subsequent to working length determination with and without integrated apex locator endomotor: Randomized control trial. [PDF]
Rawat G +5 more
europepmc +1 more source
Comparison of Digital Radiography, Conventional Film and Self-Developing Film for Working Length Determination. [PDF]
Raees Sameye M +3 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Comparative evaluation of the accuracy of six different apex locators in working length determination of molars using intraoral periapical radiographs: An in vivo study. [PDF]
Sayed A +5 more
europepmc +1 more source
Comparison of the accuracy of conventional and digital radiography in root canal working length determination: An invitro study. [PDF]
Yaghooti Khorasani MM, Ebrahimnejad H.
europepmc +1 more source
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
Comparative evaluation of efficacy in working length determination: Radiography versus Electronic apex locators using in vivo and ex vivo methods with stereomicroscope validation. [PDF]
Saha D +4 more
europepmc +1 more source

