pcEgr-hp53辐射诱导表达载体的构建及其体外抗肿瘤的作用
目的构建辐射诱导表达载体pcEgr-hp53,并研究其体外稳定转染联合X射线照射对人卵巢癌SKOV3细胞增殖的抑制作用及其凋亡的影响。方法将野生型p53cDNA全长插入pcDNA3.1质粒的多克隆位点,用Egr-1的启动子替代pcDNA3.1质粒的CMV启动子,构建成pcEgr-hp53重组质粒;通过脂质体介导转染法将重组质粒pcEgr-hp53和pcDNA3.1分别转染入SKOV3细胞株,细胞株命名为SKOV3-hp53及SKOV3-vect,并经G418筛选稳定克隆并扩增培养 ...
董丽华 +4 more
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