Results 51 to 60 of about 2,737,910 (293)

Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals

Journal of Applied Crystallography, 2016
This article reports the incorporation of a fast continuous grid scan with both still and oscillation images into the Swiss Light Source macromolecular crystallography beamlines and its application in visualization of protein crystals with scanning ...
J. Wojdyla, E. Panepucci, I. Martiel, S. Ebner, Chia-Ying Huang, M. Caffrey, O. Bunk, Meitian Wang   +7 more
semanticscholar   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Nano-printed miniature compound refractive lens for desktop hard x-ray microscopy. [PDF]

PLoS ONE, 2018
Hard x-ray lenses are useful elements in x-ray microscopy and in creating focused illumination for analytical applications such as x-ray fluorescence imaging. Recently, polymer compound refractive lenses for focused illumination in the soft x-ray regime (
Mona Mirzaeimoghri, Alejandro Morales Martinez, Alireza Panna, Eric E Bennett, Bertrand M Lucotte, Don L DeVoe, Han Wen   +6 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

AI-driven microscopy: from classical analysis to deep learning applications

Methods in Microscopy
Microscopy has revolutionized life sciences by enabling detailed visualization of cellular and subcellular processes. Recent advancements in microscope technology have enhanced our ability to capture complex biological events, generating vast amounts of ...
Bhattiprolu Sreenivas
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Structural, Interfacial, and Electrochemical Stability of La_0.3Ca_0.7Fe_0.7Cr_0.3O_3-δ Electrode Material for Application as the Oxygen Electrode in Reversible Solid Oxide Cells

Crystals, 2022
A detailed study aimed at understanding the structural, interfacial, and electrochemical performance stability of La0.3Ca0.7Fe0.7Cr0.3O3-δ (LCFCr) electrode material for application as the oxygen electrode in reversible solid oxide cells (RSOCs) is ...
Haris Masood Ansari, David Avila-Brande, Steve Kelly, Paul Kwesi Addo, Beatriz Molero-Sánchez   +4 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Impact of Sample Preparation Methods on Single-Cell X-ray Microscopy and Light Elemental Analysis Evaluated by Combined Low Energy X-ray Fluorescence, STXM and AFM

Molecules, 2023
Background: Although X-ray fluorescence microscopy is becoming a widely used technique for single-cell analysis, sample preparation for this microscopy remains one of the main challenges in obtaining optimal conditions for the measurements in the X-ray ...
Lucia Merolle, Lorella Pascolo, Luisa Zupin, Pietro Parisse, Valentina Bonanni, Gianluca Gariani, Sasa Kenig, Diana E. Bedolla, Sergio Crovella, Giuseppe Ricci, Stefano Iotti, Emil Malucelli, George Kourousias, Alessandra Gianoncelli   +13 more
doaj   +1 more source

Soft X-Ray Microscopy Radiation Damage On Fixed Cells Investigated With Synchrotron Radiation FTIR Microscopy

Scientific Reports, 2015
Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and the effects of radiation damage, proposing strategies to minimise or prevent it.
Alessandra Gianoncelli, Lisa Vaccari, G. Kourousias, D. Cassese, D. Bedolla, D. Bedolla, S. Kenig, P. Storici, M. Lazzarino, Maya Kiskinova   +9 more
semanticscholar   +1 more source