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Development of Recombinant Antibody by Yeast Surface Display Technology [PDF]
Current Research in Pharmacology and Drug DiscoveryRecombinant antibodies have emerged as powerful tools in various fields, including therapeutics, diagnostics, and research applications. The selection of high-affinity antibodies with desired specificity is a crucial step in the development of recombinant antibody-based products.
Mujahed Mustafa
exaly +5 more sources
Mapping the residue specificities of epigenome enzymes by yeast surface display [PDF]
Cell Chemical Biology, 2021 Histone proteins are decorated with a combinatorially and numerically diverse set of biochemical modifications. Here, we describe a versatile and scalable approach which enables efficient characterization of histone modifications without the need for recombinant protein production.
Balaji Rao, Albert J Keung
exaly +4 more sources
Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications. [PDF]
Front Bioeng Biotechnol, 2021 Yeast surface display (YSD) is a “whole-cell” platform used for the heterologous expression of proteins immobilized on the yeast’s cell surface. YSD combines the advantages eukaryotic systems offer such as post-translational modifications, correct folding and glycosylation of proteins, with ease of cell culturing and genetic manipulation, and allows of
Teymennet-Ramírez KV +2 more
europepmc +4 more sources
Rapidly Inducible Yeast Surface Display for Antibody Evolution with OrthoRep. [PDF]
ACS Synth BiolAbstract We recently developed ‘autonomous hypermutation yeast surface display’ (AHEAD), a technology that enables the rapid generation of potent and specific antibodies in yeast. AHEAD pairs yeast surface display with an error-prone orthogonal DNA replication system (OrthoRep) to continuously and rapidly mutate surface-displayed ...
Paulk AM, Williams RL, Liu CC.
europepmc +4 more sources
Applications of Yeast Surface Display for Protein Engineering. [PDF]
Methods Mol Biol, 2015 The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications.
Cherf GM, Cochran JR.
europepmc +4 more sources
Molecular evolution of peptides by yeast surface display technology. [PDF]
Medchemcomm, 2019 This review provides a detailed analysis of the diverse genetically encoded peptides that have been evolved by using yeast surface display technology.
Linciano S +3 more
europepmc +4 more sources
Efficient yeast surface-display of novel complex synthetic cellulosomes. [PDF]
Microb Cell Fact, 2018 The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.In this study, we developed a novel synthetic cellulosome that anchors to the endogenous yeast cell wall protein a-agglutinin through disulfide bonds.
Tang H +6 more
europepmc +5 more sources
Using yeast surface display to engineer a soluble and crystallizable construct of hematopoietic progenitor kinase 1 (HPK1) [PDF]
Acta Crystallographica Section F, Structural Biology Communications, 2021 Steven Sheriff +2 more
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Yeast Surface-Displayed H5N1 Avian Influenza Vaccines [PDF]
Journal of Immunology Research, 2016 Highly pathogenic H5N1 avian influenza viruses pose a pandemic threat to human health. A rapid vaccine production against fast outbreak is desired. We report, herein, a paradigm-shift influenza vaccine technology by presenting H5N1 hemagglutinin (HA) to the surface of yeast.
Han Lei +4 more
openaire +3 more sources
Construction of HGF-Displaying Yeast by Cell Surface Engineering
Microorganisms, 2022 Hepatocyte growth factor (HGF) has been investigated as a regulator for immune reactions caused by transplantation and autoimmune diseases and other biological functions. Previous studies demonstrated that cDNA-encoding HGF administration could inhibit acute graft-versus-host disease (GVHD) after treatment via hematopoietic stem cell transplantation ...
Seiji Shibasaki +6 more
openaire +3 more sources