Results 71 to 80 of about 1,436,627 (266)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Study on Ecological Scheduling of the Xiaolangdi Reservoir Based on the Ecological Needs of Estuarine Fishes

MATEC Web of Conferences, 2018
The total water amount into the Yellow River estuary is significantly reduced with the construction of a series of reservoirs in the Yellow River, which has a lot of adversely effects on the fishes and fishery resources in the estuary.
Tian Shimin, Zhao Yi, Wang Yuanjian, Jiang Enhui, Yu Shoubing   +4 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

One-Step Combustion Synthesis of Carbon-Doped BiVO₄ Yellow Pigments with Enhanced Visible-Light Photocatalytic Antibacterial Performance

Molecules
To integrate high chromaticity with visible-light-driven antibacterial functionality in yellow inorganic pigments, carbon-doped BiVO4 (C-BiVO4) pigments were synthesized via a one-step self-propagating combustion synthesis (SCS) using citric acid as a ...
Xiaojun Zhang, Tianxu Wang, Feng Jiang, Xiaoli Su, Xun Liu, Yanqiao Xu, Guo Feng, Qian Wu   +7 more
doaj   +1 more source

Analysis on the contribution rate of driving factors for the annual water and sediment variations at the Toudaoguai hydrological station in the Yellow River

Shui kexue jinzhan, 2015
Based on those observing series of annual water and sediment volumes from 1950 to 2010,a nonlinear response model was built statistically at the Toudaoguai gauging station in the Upper Yellow River in this paper.
RAN Dachuan, YAO Wenyi, SHEN Zhenzhou, NI Yongxin, DONG Feifei   +4 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Yellow meconium

Forensic Science, Medicine and Pathology
Abstract The intestinal content of newborns is known as meconium, usually described as a black-greenish slimy substance. However, in rare cases, meconium might be yellow. This is of great relevance with regard to the forensic assessment of life birth, i.e. (longer) survival time and possible (breast-)feeding before death.
Preuß-Wössner, Johanna, Sperhake, Jan-Peter, Madea, Burkhard   +2 more
openaire   +4 more sources