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Cells in Microfluidics

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Microfluidics

Part of the book series: Topics in Current Chemistry ((TOPCURRCHEM,volume 304))

Abstract

Microfluidic devices offer a realistic environment for cell cultures as it is related to scales found in biological systems. The aim is to create more in vivo like systems, in comparison to 2D plate cultures. Creating 3D cell culture constructs increase the cell’s functionality. By controlling the microenvironment (e.g., cell matrix, flow rate, temperature) cell functionality can be increased even more. As microfluidic devices allow for precise control of the microenvironment, they are a paramount tool to study stem cells and their differentiation caused by external factors. We will give an overview of the use of microfluidic devices for some biological problems, and especially as a cell culture platforms. We focus on 3D cell cultures and stem cells and their microenvironment.

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Abbreviations

μCCA:

Microscale cell culture analogue

3D-μFCCS:

Three-dimensional microfluidic cell culture system

ALP:

Alkaline phosphatase

bFGF:

Basic fibroblast growth factor

BMSC:

Bone marrow stem cell

BSA:

Bovine serum albumin

cDNA:

Complementary deoxyribonucleic acid

CEPC:

Circulating endothelial progenitor cell

CNS:

Central nervous system

CTC:

Circulating tumour cell

DC:

Direct current

ECM:

Extracellular matrix

EGF:

Epidermal growth factor

EPC:

Endothelial progenitor cell

ESC:

Embryonic stem cell

FACS:

Fluorescence-activated cell sorter

FBS:

Fetal bovine serum

FITC:

Fluorescein isothiocyanate

FSS:

Flow shear stress

GFP:

Green fluorescent protein

hESC:

Human embryonic stem cell

hNSC:

Human neural stem cell

HSC:

Haematopoietic stem cell

IC50 :

50% maximal inhibitory concentration

iPS:

Induced pluripotent stem

mRNA:

Messenger ribonucleic acid

MSC:

Mesenchymal stem cells

PDGF:

Platelet-derived growth factor

PDMS:

Polydimethylsiloxane

PEG:

Polyethylene glycol

PZT:

Piezoelectric transducer

SMC:

Smooth muscle cell

TGF:

Transforming growth factor

UHTP:

Ultrahigh-throughput

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Authors and Affiliations

  1. Division of Nanobiotechnology, AlbaNova University Center, Royal Institute of Technology, 106 91, Stockholm, Sweden

    Chi Zhang

  2. MechanoBiology Institute, Singapore, National University of Singapore, T-Lab, 5A Engineering Drive 1, 117411, Singapore, Singapore

    Danny van Noort

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Correspondence to Danny van Noort .

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  1. The Chinese Academy of Sciences, Laboratory of Biotechnology, Dalian Institute of Chemical Physics, ZhongShan Road 457, Dalian, 116023, China, People's Republic

    Bingcheng Lin

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Zhang, C., van Noort, D. (2011). Cells in Microfluidics. In: Lin, B. (eds) Microfluidics. Topics in Current Chemistry, vol 304. Springer, Berlin, Heidelberg. https://doi.org/10.1007/128_2011_147

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