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Chemolithotrophy in Rhizobium

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Genetic Engineering of Symbiotic Nitrogen Fixation and Conservation of Fixed Nitrogen

Abstract

The first pure cultures of Rhizobium species were described by Beijerinck in 1888 who surface disinfected nodules from Vicia, Lathrus and Trifolium and cultured the nodule endophytes on gelatin plates containing plant extracts, sucrose, and asparagine. Since this pioneering discovery, Rhizobium species have been considered chemoorganotrophs and always have been supplied with carbon substrates such as hexoses, pentoses, or complex carbohydrates (Vincent, 1978).

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References

  • Albrecht, S. L., Maier, R. J., Hanus, F. J., Russell, S. A., Emerich, D. W., and Evans, H. J., 1979, Hydrogenase in R. japonicum increases nitrogen fixation by nodulated soybeans, Science, 203:1255.

    Article  PubMed  CAS  Google Scholar 

  • Anand, S. R., and Krasna, A. I., 1965, Catalysis of the H2-HTO exchange by hydrogenase: A new assay for hydrogenase, Biochemistry, 4:2747.

    Article  PubMed  CAS  Google Scholar 

  • Anderson, K., Shanmugam, K. T., Lim, S. T., Csonka, L. N., Tait, R., Hennecke, H., Scott, D. B., Hom, S. S. M., Haury, J. F., Valentine, A., and Valentine, R. C., 1980, Genetic engineering in agriculture with emphasis on nitrogen fixation, Trends in Biochem. Sci., 5:35.

    Article  Google Scholar 

  • Arp, D. J., and Burris, R. H., 1979, Purification and properties of the particulate hydrogenase from the bacteroids of soybean root nodules, Biochim. Biophys. Acta, 570:221.

    PubMed  CAS  Google Scholar 

  • Beijerink, M. W., 1888, Die Bacterien der Papilionaceenknöilchen, Botanische Zeitung, 45:725.

    Google Scholar 

  • Bethlenfalvay, G. J., and Phillips, D. A., 1979, Variation in nitrogenase and hydrogenase activity of Alaska pea root nodules, Plant Physiol., 63:816.

    Article  PubMed  CAS  Google Scholar 

  • Christeller, J. T., Laing, W. A., and Sutton, W. D., 1977, Carbon dioxide fixation by lupin root nodules. I. Characterization, association with phosphoenolpyruvate carboxylase, and correlation with nitrogen fixation during nodule development, Plant Physiol., 60:47.

    Article  PubMed  CAS  Google Scholar 

  • DeHertogh, A. A., Mayeux, P., and Evans, H. J., 1964, The relationship of cobalt requirement to propionate metabolism in Rhizobium, J. Biol. Chem., 239:2446.

    PubMed  CAS  Google Scholar 

  • Dixon, R. O. D., 1968, Hydrogenase in pea root nodule bacteroids, Arch. Microbiol., 62:272.

    Article  CAS  Google Scholar 

  • Dixon, R. O. D., 1972, Hydrogenase in legume root nodule bacteroids: Occurrence and properties, Arch. Microbiol., 85:193.

    Article  CAS  Google Scholar 

  • Dixon, R. O. D., 1978, Nitrogenase-hydrogenase interrelationships in rhizobia, Biochimie., 60:233.

    Article  PubMed  CAS  Google Scholar 

  • Emerich, D. W., Albrecht, S. L., Russell, C. A., Ching, T. M., and Evans, H. J., 1980a, Oxyleghemoglobin-mediated H2 oxidation by R. japonicum. USDA 122 DES bacteroids, Plant Physiol., 65:605.

    Article  PubMed  CAS  Google Scholar 

  • Emerich, D. W., Ruiz-Argüeso, T., Ching, T. M., and Evans, H. J., 1979, Hydrogen-dependent nitrogenase activity and ATP formation in R. japonicum bacteroids, J. Bacteriol., 137:153.

    PubMed  CAS  Google Scholar 

  • Emerich, D. W., Ruiz-Argüeso, T., Russell, S. A., and Evans, H. J., 1980b, Investigations of the H2 oxidation system in Rhizobium japonicum (122 DES) nodule bacteroids, Plant Physiol., accepted.

    Google Scholar 

  • Evans, H. J., Emerich, D. W., Lepo, J. E., Maier, R. J., Carter, K. R., Hanus, F. J., and Russell, S. A., 1980a, The role of hydrogenase in nodule bacteroids and free-living rhizobia, in: “International Symposium on Nitrogen Fixation,” W. D. P. Stewart and J. R. Gallon, eds., Academic Press, London, in press.

    Google Scholar 

  • Evans, H. J., Emerich, D. W., Ruiz-Argüeso, T., Albrecht, S. L., Maier, R. J., Simpson, F., and Russell, S. A., 1978, Hydrogen metabolism in legume nodules and rhizobia: Some recent developments, in: “Hydrogenases: Their Catalytic Activity, Structure, and Function,” H. G. Schlegel and K. Schneider, eds., Erich Goltze KG, Gottingen.

    Google Scholar 

  • Evans, H. J., Emerich, D. W., Ruiz-Argüeso, T., Maier, R. J., and Albrecht, S. L., 1980b, Hydrogen metabolism in the legume rhizobium symbiosis, in: “Nitrogen Fixation: Symbiotic Associations and Cyanobacteria,” Vol. II, W. H. Orne-Johnson and W. E. Newton, eds., University Park Press, Baltimore.

    Google Scholar 

  • Hanus, F. J., Maier, R. J., and Evans, H. J., 1979, Autotrophic growth of H2−uptake positive strains of R. japonicum in an atmosphere supplied with hydrogen gas, Proc. Natl. Acad. Sci., 76:1788.

    Article  PubMed  CAS  Google Scholar 

  • Jackson, E. K., and Evans, H. J., 1966, Propionate in heme biosynthesis in soybean nodules, Plant Physiol., 41:1673.

    Article  PubMed  CAS  Google Scholar 

  • Laing, W. A., Christeller, J. T., and Sutton, W. D., 1979, Carbon dioxide fixation by lupin root nodules. II. Studies with 14C−labeled glucose, the pathway of glucose catabolism and the effects of some treatments that inhibit nitrogen fixation, Plant Physiol., 63:450.

    Article  PubMed  CAS  Google Scholar 

  • Lawrie, A. C., and Wheeler, C. T., 1975, Nitrogen fixation in the root nodules of Vicia faba L. in relation to the assimilation of carbon. I. Plant growth and metabolism of photosynthetic assimilates, New Phytologist, 74:429.

    Article  CAS  Google Scholar 

  • Lepo, J. E., Hanus, F. J., and Evans, H. J., 1980, Further studies on the chemautotrophic growth of hydrogen uptake positive strains of R. japonicum, J. Bacteriol., 141:664.

    PubMed  CAS  Google Scholar 

  • Lim, S. T., 1978, Determination of hydrogenase in free-living cultures of Rhizobium japonicum and energy efficiency of soybean nodules, Plant Physiol., 62:609.

    Article  PubMed  CAS  Google Scholar 

  • Lim, S. T., Hennecke, H., and Scott, D. B., 1969, Effect of cyclic guanosine 3′, 5′-monophosphate on nitrogen fixation in R. japonicum, J. Bacteriol., 139:256.

    Google Scholar 

  • Lim, S. T., and Shanmugam, K. T., 1969, Regulation of hydrogen utilization in Rhizobium japonicum by cyclic AMP, Biochim. Biophys. Acta, 584:479.

    Article  Google Scholar 

  • Lowe, R. H., and Evans, H. J., 1962, Carbon dioxide requirement for growth of legume nodule bacteria, Soil Sci., 94:351.

    Article  CAS  Google Scholar 

  • Maier, R. J., 1980, Rhizobium japonicum mutant strains unable to grow chemoautotrophically with H2, in: “Abstracts of the Annual Meeting of the American Society for Microbiology,” R. A. Finkelstein, ed., American Society of Microbiology, Washington, DC.

    Google Scholar 

  • Maier, R. J., Campbell, N. E. R., Hanus, F. J., Simpson, F. B., Russell, S. A., and Evans, H. J., 1978a, Expression of hy-drogenase activity in free-living R. japonicum, Proc. Natl. Acad. Sci., 75:3258.

    Article  PubMed  CAS  Google Scholar 

  • Maier, R. J., Hanus, F. J., and Evans, H. J., 1969, Regulation of hydrogenase in R. japonicum, J. Bacteriol., 137:824.

    Google Scholar 

  • Maier, R. J., Postgate, J. R., and Evans, H. J., 1978b, Mutants of R. japonicum unable to utilize hydrogen, Nature, 276:494.

    Article  CAS  Google Scholar 

  • Maruyama, Y., 1975, Studies on symbiotic nitrogen fixation: Nitrogen-fixing and hydrogenase activities in extracts of legume root nodules, in: “Nitrogen Fixation and Nitrogen Cycle,” H. Takahashi, ed., University of Tokyo Press, Tokyo.

    Google Scholar 

  • Maruyama, Y., Onodera, K., and Funahashi, S., 1967, Nitrogen and hydrogen fixation in extracts of legume root nodules, in: “Abstracts of VII Intern. Congr. Biochem.,” J52, Tokyo.

    Google Scholar 

  • McCrae, R. E., Hanus, F. J., and Evans, H. J., 1978, Properties of the hydrogenase system in Rhizobium japonicum bacteroids, Biochem. Biophys. Res. Comm., 80:384.

    Article  PubMed  CAS  Google Scholar 

  • Mulder, E. G., and Veen, W. L. van, 1960, The influence of carbon dioxide on symbiotic nitrogen fixation, Plant and Soil, 13:265.

    Article  CAS  Google Scholar 

  • Peterson, J. B., and Evans, H. J., 1979, Phosphoenolpyruvate carboxylase from soybean nodule cytosol: Evidence for isoenzymes and kinetics of the most active component, Biochim. Biophys. Acta, 567:445.

    PubMed  CAS  Google Scholar 

  • Phelps, A. S., and Wilson, P. W., 1941, Occurrence of hydrogenase in nitrogen fixing organisms, Proc. Soc. Exp. Biol., 47:473.

    CAS  Google Scholar 

  • Probst, I., and Schlegel, H. G., 1976, Respiratory components and oxidase activities in Alcaligenes eutrophus, Biochim. Biophys. Acta, 440:412.

    Article  PubMed  Google Scholar 

  • Purohit, K., and Evans, H. J., 1980, D-Ribulose 1, 5-bisphosphate carboxylase/oxygenase from autotrophically grown Rhizobium japonicum, in: “Abstracts of American Society of Plant Physiologists Meeting at Washington State University, Pullman, Washington,” American Society of Plant Physiologists, Bethesda.

    Google Scholar 

  • Rittenberg, S. C., 1969, The roles of exogenous organic matter in the physiology of chemolithotrophic bacteria, in: “Advances in Microbial Physiology,” Vol. 3, A. H. Rose and J. F. Wilkinson, eds., Academic Press, London.

    Google Scholar 

  • Ruiz-Argüeso, T., Emerich, D. W., and Evans, H. J., 1969, Characteristics of the hydrogen oxidizing system in soybean nodule bacteroids, Arch. Microbiol., 121:199.

    Article  Google Scholar 

  • Ruiz-Argüeso, T., Hanus, F. J., and Evans, H. J., 1978, Hydrogen production and uptake by pea nodules as affected by strains of Rhizobium leguminosarum, Arch. Microbiol., 116:113.

    Article  Google Scholar 

  • Schink, B., and Schlegel, H. G., 1978, Hydrogen metabolism in aerobic hydrogen-oxidizing bacteria, Biochimie, 60:297.

    Article  PubMed  CAS  Google Scholar 

  • Schlegel, H. G., and Eberhardt, U., 1972, Regulatory phenomena in the metabolism of Knallgasbacteria, in: “Advances in Microbial Physiology,” Vol. 7, A. H. Rose and D. W. Tempest, eds., Academic Press, London.

    Google Scholar 

  • Schubert, K. R., Engelke, J. A., Russell, S. A., and Evans, H. J., 1977, Hydrogen reactions of nodulated leguminous plants. I. Effect of rhizobial strain and plant age, Plant Physiol., 60:651.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, K. R., and Evans, H. J., 1976, Hydrogen evolution: A major factor affecting the efficiency of nitrogen fixation in nodulated symbionts, Proc. Natl. Acad. Sci., 73:1207.

    Article  PubMed  CAS  Google Scholar 

  • Schubert, K. R., and Evans, H. J., 1977, The relation of hydrogen reactions to nitrogen fixation in nodulated symbionts, in: “Proceedings of the II International Symposium on N2 Fixation: Recent Developments in N2 Fixation,” W. E. Newton, J. R. Postgate, and C. Rodriguez-Barrueco, eds., Academic Press, London.

    Google Scholar 

  • Schubert, K. R., Jennings, N. T., and Evans, H. J., 1978, Hydrogen reactions of nodulated leguminous plants. II. Effects on dry matter accumulation and nitrogen fixation, Plant Physiol., 61:398.

    Article  PubMed  CAS  Google Scholar 

  • Scott, D. B., Farnden, K. J. F., and Robertson, J. G., 1976, Ammonia assimilation in lupin nodules, Nature, 263:703.

    Article  CAS  Google Scholar 

  • Simpson, F. B., Maier, R. J., and Evans, H. J., 1979, Hydrogen-stimulated CO2 fixation and coordinate induction of hydrogen-ase and ribulosebisphosphate carboxylase in an H2−uptake positive strain of R. japonicum, Arch. Microbiol., 123:1.

    Article  CAS  Google Scholar 

  • Turner, G. L., and Bergersen, F. J., 1969, The relationship between nitrogen fixation and the production of HD from D2 by cell-free extracts of soya-bean nodule bacteroids, Biochem. J., 115:529.

    PubMed  CAS  Google Scholar 

  • Valley, G., and Rettger, L. F., 1927, The influence of carbon dioxide on bacteria, J. Bacteriol., 14:101.

    PubMed  CAS  Google Scholar 

  • Vincent, J. M., 1977, Rhizobium: General microbiology, In: “A Treatise on Dinitrogen Fixation, Section III,” R. W. F. Hardy and W. S. Silver, eds., John Wiley and Sons, New York.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis, OR, 97331, USA

    H. J. Evans, J. E. Lepo, F. J. Hanus, K. Purohit & S. A. Russell

Authors
  1. H. J. Evans
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  2. J. E. Lepo
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  3. F. J. Hanus
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  4. K. Purohit
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  5. S. A. Russell
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Editor information

Editors and Affiliations

  1. University of California, Davis, California, USA

    J. M. Lyons , R. C. Valentine , D. A. Phillips , D. W. Rains  & R. C. Huffaker , , ,  & 

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© 1981 Plenum Press, New York

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Evans, H.J., Lepo, J.E., Hanus, F.J., Purohit, K., Russell, S.A. (1981). Chemolithotrophy in Rhizobium . In: Lyons, J.M., Valentine, R.C., Phillips, D.A., Rains, D.W., Huffaker, R.C. (eds) Genetic Engineering of Symbiotic Nitrogen Fixation and Conservation of Fixed Nitrogen. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3953-3_14

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  • DOI: https://doi.org/10.1007/978-1-4684-3953-3_14

  • Publisher Name: Springer, Boston, MA

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