Abstract
In this chapter, we will learn to:
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Further Reading
Hermann R, Lehmann M, Buchs J (2003) Characterization of gas-liquid mass transfer phenomena in microtiter plates. Biotechnol Bioeng 81(2):178–186
Kong F, Yuan L, Zheng YF, Chen W (2012) Automatic liquid handling for life science: a critical review of the current state of the art. J Lab Autom 17(3):169–185
Trask OJ (2018) Guidelines for microplate selection in high content imaging. Methods Mol Biol 1683:75–88
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Exercise
Exercise
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(a)
Calculate the mean, standard deviation and coefficient of variation of the three internal controls from library plate A (◘ Fig. 9.14).
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(b)
Repeat the calculations for library plate B.
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(c)
What information could you deduce from the calculations in (a) and (b)?
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(d)
Do you observe edge effect in the library plates? Justify your answer.
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(e)
Which library plate is prepared using a higher mutation rate? Justify your answer.
Assay data from two library plates A and B. The values for internal controls are coloured in red.
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Wong, T.S., Tee, K.L. (2020). High-Throughput Screening (HTS). In: A Practical Guide to Protein Engineering . Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-56898-6_9
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DOI: https://doi.org/10.1007/978-3-030-56898-6_9
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