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Cytometrie Tout Terrain or Bush DNA Cytometry

  • Conference paper
Flow Cytometry

Part of the book series: NATO ASI Series ((ASIH,volume 67))

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  • 3 Citations

Abstract

This unconventional title derives partially from our subject matter being flow cytometry applied to plants, but principally from a proposition developed in the conclusion: namely, that an industrial application of cytometry in the diagnosis of plant ploidy levels will be vastly more economic when the cytometer can go to the plants — on a wheel-barrow if necessary — rather than the samples coming to the laboratory!

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References

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9: 208–218

    Article  CAS  Google Scholar 

  • Arumuganathan K, Slattery JP, Tanksley SD, Earle ED (1991) Preparation and flow cytometric analysis of metaphase chromosomes of tomato. Theor Appl Genet 82: 101–111

    Article  Google Scholar 

  • Bennett MD, Smith JB (1975) Nuclear DNA amounts in Angiosperms. Philos Trans R Soc, Ser B 274: 227–274

    Article  Google Scholar 

  • Bennett MD, Smith JB, Heslop-Harrison JS (1982) Nuclear DNA amounts in Angiosperms. Proc R Soc Biol 216: 179–199

    Article  CAS  Google Scholar 

  • Berglund DL, Strobel S, Sugawara F, Strobel GA (1988) Flow cytometry as a method for assaying the biological activity of phytotoxins. Plant Sci 56: 183–188

    Article  CAS  Google Scholar 

  • Bergounioux C, Brown S (1989) Plant cell cycle analysis with isolated nuclei. In: Crissman HA, Darzynkiewicz Z (ed) Flow Cytometry, Academic Press, New York (Methods in Cell Biology, 33, 563 )

    Chapter  Google Scholar 

  • Bergounioux C, Brown SC, Petit P (1992) Flow cytometry and plant protoplast cell biology. Physiol Plant, in press

    Google Scholar 

  • Bergounioux C, Perennes C, Brown SC, Gadal P (1988a) Nuclear RNA quantification in protoplast cell-cycle phases. Cytometry 9: 84–87

    Article  PubMed  CAS  Google Scholar 

  • Bergounioux C, Perennes C, Brown SC, Sarda C, Gadal P (1988b) Relation between protoplast division, cell cycle stage and nuclear chromatin structure. Protoplasma 142: 127–136

    Article  CAS  Google Scholar 

  • Brown SC, Bergounioux C (1989) Plant flow cytometry. In: Yen A (ed) Flow Cytometry: Advanced research and clinical applications, vol II. CRC Press, Boca Raton Florida, p 195

    Google Scholar 

  • Brown, S, Bergounioux, C, Tallet, S, Marie, D (1991a) Flow cytometry of nuclei for ploidy and cell cycle analysis. In: Negrutiu I, Gharti-Chherti GB (ed) A laboratory guide for cellular and molecular plant biology. Birkhäuser Verlag, Basel, p 326

    Google Scholar 

  • Brown SC, Devaux P, Marie D, Bergounioux C, Petit P (1991b) Cytométrie en flux: Application à l’analyse de la ploïdie chez les végétaux. Biofutur 105 (October): 1–14 (Technoscope n°47)

    Google Scholar 

  • Brown SC, Renaudin JP, Prevot C, Guern J (1984) Flow cytometry and sorting of plant protoplasts: Technical problems and physiological results from a study of pH and alkaloids in Catharanthus roseus. Phyiol Vég 22: 541–554

    CAS  Google Scholar 

  • Chaput MH, Sihachakr D, Ducreux G, Marie D, Barghi N (1990) Somatic hybrid plants produced by electrofusion between dihaploid potatoes: BF15 (Hl), Aminca (H6) and Cardinal ( H3 ). Plant Cell Rep 9: 411–414

    Google Scholar 

  • Conia J, Muller P (1989) Isolation, classification, and flow cytometric sorting of plant chromosomes. In: Yen A (ed) Flow Cytometry: Advanced research and clinical applications, vol I. CRC Press, Boca Raton Florida, p 143

    Google Scholar 

  • Costich DE, Meagher TR, Yurkow EJ (1991) À rapid means of sex identification in Silene latifolia by use of flow cytometry. Plant Mol Biol Rep 9: 359–370

    Article  Google Scholar 

  • De Laat AM, Gohde W, Vogekzang MJ (1987) Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303–307

    Article  Google Scholar 

  • De Rocher EJ, Harkins KR, Galbraith DW, Bohnert HJ (1990) Developmentally regulated systematic endopolyploidy in succulents with small genomes. Science 250: 99–101

    Article  PubMed  Google Scholar 

  • Deslauriers C, Powell AD, Fuchs K, Pauls KP (1991) Flow cytometric characterization and sorting of cultured Brassica napus microspores. Biochim Biophys Acta 1091: 165–172

    Article  PubMed  CAS  Google Scholar 

  • Fahleson J, Dixelius J, Sundberg E, Glimelius K (1988) Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae. Plant Cell Rep 7: 74–77

    Article  CAS  Google Scholar 

  • Fox MH, Galbraith DW (1990) Application of flow cytometry and sorting to higher plant systems. In: Melamed M, Lindmo T, Mendelsohn M (ed) Flow cytometry and sorting, 2nd edn. Wiley-Liss, New York, p 632

    Google Scholar 

  • Galbraith DW (1989) Analysis of higher plants by flow cytometry and cell sorting. Int Rev Cytol 116: 165–228

    Article  Google Scholar 

  • Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051

    Article  PubMed  CAS  Google Scholar 

  • Gantet P, Hubac C, Brown SC (1990) Flow cytometric fluorescences anisotropy of lipophilic probes in epidermal and mesophyll protoplasts from water-stressed Lupinus albus L. Plant Physiol 94: 729–737

    Article  PubMed  CAS  Google Scholar 

  • Godelle B, Cartier D, Marie D, Brown SC, Siljak-Yakovlev S (1990) Relationship between flow cytometric determination of nuclear DNA content and the amount of heterochromatin within the Crepis praemorsa complex. Biol Cell 70 (1–2): 28a

    Google Scholar 

  • Godelle B, Cartier D, Marie D, Brown S, Siljak-Yakovlev S (1992) Heterochromatin study reveals non-linearity of fluorometry for genomic base composition, pers. comm.

    Google Scholar 

  • Hammatt N, Lister A, Blackhall NW, Gartland J, Ghose TK, Gilmour DM, Power JB, Davet MR, Cocking EC (1990) Selection of plant heterokaryons from diverse origins by flow cytometry. Protoplasma 154: 34–44

    Article  Google Scholar 

  • Harkins KR, Galbraith DW (1987) Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry 8:60-70 Langlet O (1927) Svensk Bot Tids 21: 397–422

    Google Scholar 

  • Le Gall Y, Brown S, Marie D, Mejjad M, Kloareg B (1992) Quantification of nuclear DNA and GC% in marine macroalgae by flow cytometry of isolated nuclei. J Phycology in press

    Google Scholar 

  • Lister A (1989) Flow cytometry for selection of plant cells in vitro. In: Dix PJ (ed) Selection of plant cells, VCH Verlagsgesellschaft, Weinheim, chpt 3

    Google Scholar 

  • Matine A (1990) Biosynthèse d’isoprenoïdes par des protoplastes et plastes isolés du fruit de calamondin. Université de Bordeaux I, Ph.D. thesis

    Google Scholar 

  • Perennes C, Bergounioux C, Gadal P (1990) Direct assessment of hormone stimulated transcription in protoplasts and isolated nuclei of Petunia hybrida. Plant Cell Rep 8: 684–686

    Article  CAS  Google Scholar 

  • Petit PX (1992) Flow cytometric analysis of Rhodamine 123 fluorescence during modulation of the membrane potential in plant mitochondria. Plant Physiol 98: 279–286

    Article  PubMed  CAS  Google Scholar 

  • Petit PX, Conia J, Brown SC, Bergounioux C (1986) Cytométrie en flux et biotechnologies végétales. Biofutur 5 (November): 128–137

    Google Scholar 

  • Petit PX, Diolez P, de Kouchkovsky Y (1989) Flow cytometric analysis of energy transducing organelles: mitochondria and chloroplasts. In: Yen A (ed) Flow Cytometry, Advanced research and clinical applications, vol I. CRC Press, Boca Raton Florida, p 271

    Google Scholar 

  • Sangwan R, Bourgeois Y, Brown SC, Vasseur G, Sangwan-Norreel B (1992) Characterization of competent cells and early events of Agrobacterium mediated genetic transformation in Arabidopsis thaliana. Planta, in press

    Google Scholar 

  • Schröder W, Petit PX (1990) Study of lectin-binding sites on the chloroplast envelope using flow cytometry analysis. Physiol Plant 79 (2, part 2): A59

    Google Scholar 

  • Sgorbati S, Levi M, Sparvoli E, Trezzi F, Lucchini G (1986) Static and flow cytometry of 4’,6-diamidino-2-phenylindole ( DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiol Plantarum 68: 471–476

    Article  CAS  Google Scholar 

  • Sgorbati S, Sparvoli E, Levi M, Chiatante D (1989) Bivariate cytofluorimetric analysis of nuclear protein and DNA relative to cell kinetics during germination of Pisum sativum seed. Physiol Plantarum 75: 479–484

    Article  CAS  Google Scholar 

  • Sree Ramulu K, Dijkhuis P (1986) Flow cytometric analysis of polysomaty and in vitro genetic instability in potato. Plant Cell Rep 3: 234–237

    Article  Google Scholar 

  • Tagu D, Bergounioux C, Perennes C, Brown SC, Muller P, Gadal P (1987) Analysis by flow cytometry of the primary events of Petunia hybrida protoplast transformation. Plant Sci 51: 215–223

    Article  CAS  Google Scholar 

  • Ulrich I, Fritz B, Ulrich W (1988) Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Sci 55: 151–158

    Article  CAS  Google Scholar 

  • Ulrich I, Ulrich W (1991) High-resolution flow cytometry of nuclear DNA in higher plants. Protoplasma 165: 212–215

    Article  Google Scholar 

  • Veuskens J, Marie D, Hinnisdeals S, Brown S (1992) Flow cytometry and sorting of plant chromosomes. In: Radbruch A (ed) EMBO Course Methods, Flow cytometry and cell sorting, Springer, Berlin Heidelberg New York (Monograph Series, Methods in biology and medicine), in press

    Google Scholar 

  • Xu C, Auger J, Givindjee (1990) Chlorophyll a fluorescence measurements of isolated spinach thylakoids obtained by using single-laser-based flow cytometry. Cytometry 11: 349–358

    Article  PubMed  CAS  Google Scholar 

  • Ye D, Oliveira M, Veuskens J, Wu Y, Installe P, Hinnisdaels S, Truong AT, Brown SC, Mouras A, Negrutiu I (1991) Sex determination in the dioecious Melandrium. The X/Y chromosome systems allows complementary cloning strategies. Plant Sci 80: 93–106

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Cytométrie Institut des Sciences Végétales, Centre National de la Recherche Scientifique, 91198, Gif-sur-Yvette, France

    Spencer C. Brown

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  1. Spencer C. Brown
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Editor information

Editors and Affiliations

  1. Laboratoire de Cytométrie, Centre National de la Recherche Scientifique, B. P. 3, 94801, Villejuif, France

    Alain Jacquemin-Sablon

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© 1993 Springer-Verlag Berlin Heidelberg

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Brown, S.C. (1993). Cytometrie Tout Terrain or Bush DNA Cytometry. In: Jacquemin-Sablon, A. (eds) Flow Cytometry. NATO ASI Series, vol 67. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84616-8_15

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  • DOI: https://doi.org/10.1007/978-3-642-84616-8_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84618-2

  • Online ISBN: 978-3-642-84616-8

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