Method for Chemical Separation
Chromatography is a general term describing chemical procedures for the separation of mixtures of elements or compounds. Chromatographic methods are used for both preparation and analysis of samples. A fundamental principle of chromatography is that components of a mixture will have different distribution or partition coefficients between a mobile and stationary phase. Distribution coefficients are a measure of a compounds “preference” for either the mobile or the stationary phase. A solution containing compounds to be separated is usually dissolved in the mobile phase, which then is allowed to flow through a stationary phase. The dissolved components elute at different times in this flow system, the times being determined by the distribution coefficients – those that are preferentially retained by the stationary phase appear later in the chromatogram, which is the graphical output of the chromatographic separation.
Preparative chromatography is part of...
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Wehmiller, J. (2013). Chromatography. In: Rink, W., Thompson, J. (eds) Encyclopedia of Scientific Dating Methods. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6326-5_113-3
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DOI: https://doi.org/10.1007/978-94-007-6326-5_113-3
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