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ß-barrel Channel Proteins as Tools in Nanotechnology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 794))

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Abstract

The following chapter explains how to practically transform a β-barrel membrane protein (MP) into a nano-channel with desired geometrical and/or functional features, starting from the concept-design and design of the respective gene. It will then give an overview on the conventional means of production and purification of bacterial OMPs (outer membrane proteins), stressing on the problems and challenges of over-expressing OMPs into the Gram-negative bacterial outer membrane and of isolating them from the outer membrane. Furthermore the special problems of producing modified β-barrel MPs and ways to overcome these problems by using alternative methods will be named and explained. The different ways of analyzing OMP samples regarding yield, purity and correct folding will be considered briefly and the chapter discuss the distinctive experimental adaptations to scale-up the production of OMPs in general and modified OMPs especially. As for many industrial applications of OMP derived nano-materials vast amounts of the proteins need to be produced, exceeding the capacities of conventional methods. The chapter will close with a discussion on artificial β-barrel structures to which OMPs are an alternative.

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Authors and Affiliations

  1. Sustainable Momentum, Aachen, Germany

    Marco Fioroni

  2. Department of Biology, RWTH Aachen University, Aachen, Germany

    Tamara Dworeck

  3. Center of Smart Interfaces, Technische Universität Darmstadt, Darmstadt, Germany

    Francisco Rodríguez-Ropero

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Fioroni, M., Dworeck, T., Rodríguez-Ropero, F. (2014). Biotechnology. In: ß-barrel Channel Proteins as Tools in Nanotechnology. Advances in Experimental Medicine and Biology, vol 794. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7429-2_5

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