Summary
We have been interested in studying the factors affecting the quality of food. It is important to detect toxic substances in foods as well as to control food-born disease microorganisms. We have been applied in vitro bioassay to evaluate the quality of food. We tried the following assay procedure: Alamar Blue reduction assay, lactose dehydrogenase assay, neutral red inclusion assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl2H-tetrazolium bromide(MIT) reduction assay, WST-1 reduction assay, and others. We have developed a chemiluninescent cytotoxicity assay and applied it to food component and toxins. This assay was sensible and rapid. WST-1 reduction assay was easy to operate.
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References
Doyle, M.D., Steihart, C.E. and Cochrane, B.A. (1993) Food Safety 1993. Marcel Dekker,Inc.,New York.
Frazier, J.M. (1992) In Vitro Toxicity testing. Applications to Safety Evaluation, MarcelDeller, Inc., New York 1992.
Isshiki, K., Mine, H. and Shinohara, K. (1992) Effects of some food additives and mycotoxins on the growth of HuH-6KK cells,Anisal Cell Technologp:Basic and Applied Aspects, 4. 559–563.
Murakami, H. (1989)Serum-free media used for cultivation of hybridoma. Advances in Biotechnological Process, 11. 107–141.
Page, B., Page, M., and Noel, C. (1993)A new fluorometric assay for cytotoxic measuremnets in vito. International Journal of Oncology. 3. 474–476.
Borenfreund, E., Babich, H. and Martin-Alguacil, N. (1988)Comparisons of two in vitro cytotoxicity assays-The neutral red(NR) and tetrazorium MTT tests. Toxicol. In Vitro,2,1–6.
Ally, M.C., Scudiero, D.A., Monks, A., Hursey, L., Czerwinski, M.J., Fine, D.L., Abbot, B.J., Mayo, J.G., Sheomaker, R.II, and Boyd, M.R. (1988)Feasbility of drug screening with panels of human tumor cell lines using microculture tetrazolium assay. Cancer Research. 48. 589–601.
Ishiyama, M., Shiga, M., Sasamoto, K., Mizoguchi, M. and Pin-gang, II. (1991)A new sulfonated tetrazolium salt that produces a high water-soluble formazan dye. Ches.Phars.Bull.,41,1118–1122.
Yamashoji, S., Nishimoto, F., Usuda, M., Kubota, H. and Isshiki, K. (1992) Application of the chemiluminescent assay to cytotoxicity test:Detectionof Minadione-catalyzes H202 production by Viable-catalysed 11202 production by viable cells. Anal.Bioches.207. 255–260.
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© 1995 Springer Science+Business Media Dordrecht
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Isshiki, K., Asano, M., Yamashoji, S. (1995). Cytotoxicity Testing for Food Safety Evaluation. In: Beuvery, E.C., Griffiths, J.B., Zeijlemaker, W.P. (eds) Animal Cell Technology: Developments Towards the 21st Century. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0437-1_158
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DOI: https://doi.org/10.1007/978-94-011-0437-1_158
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4195-9
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