Abstract
The genome of plants is remarkably complex and a particular DNA fragment of interest comprises only a small fraction of the whole genome. Therefore, construction of a useful and representative recombinant genomic library is dependent on the generation of a large population of clones. This is necessary to ensure that the library will contain at least one copy of every sequence of DNA in the genome. Although a common and simple way to obtain a high quality library is to purchase a stock or custom-made library from reputable commercial sources, many researchers prefer to create libraries in their own laboratories.
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References
Wahl GM, Lewis KA, Ruiz JC, Rothenberg B, Zhao J, Evans GA (1987) Cosmid vectors for rapid genomic walking, restriction mapping and gene transfer. Proc Natl Acad Sci USA 84: 2160–2164.
Alting-Mees MA, Short JM (1989) pBluescript II: Gene mapping vectors. Nucl Acids Res 17: 9494.
Frischauf AM, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170: 827–842.
Sambrook J, Fritsch, EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor, New York, NY: Cold Spring Harbor Laboratory Press.
Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (1983) Lambda II Cold Spring Harbor, New York: Cold Spring Harbor Laboratories.
Huynh TV, Young RA, Davis RW (1985) In: Glover NM (ed) DNA Cloning: A Practical Approach, Vol 1. Oxford: IRL Press.
Young, RA, Davis RW (1983) Yeast RNA Polymerase II genes: Isolation with antibody probes. Science 222: 778–782.
Short JM, Fernandez JA, Sorge JA, Huse WD (1988) Lambda ZAP: A bacteriophage lambda expression vector with in vivo excision properties. Nucl Acids Res 16: 7583–7600.
Alting-Mees MA, Short JM (1992) New lambda and phagemid vectors for prokaryotic and eukaryotic expression. Strategies 5: 58–61.
Short JM, Sorge JA (1991) In vivo excision properties of bacteriophage lambda ZAP® expression vectors. Methods Enzymol 216: 495–508.
Personal observations by Stratagene’s Custom Library Department.
Shapiro HS (1976) Handbook of Biochemistry and Molecular Biology, pp. 258–262. CRC Press Boca Raton, FL.
Raleigh EA, Wilson G (1986) Escherichia coli K-12 restricts DNA containing 5-methylcyto-sine. Proc Natl Acad Sci USA 83: 9070–9074.
Ross RK, Achberger EC, Draymer HD (1989) Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus. J Bacteriol 171: 1974–1981.
Kretz PL, Kohler SW, Short JM (1992) Identification and characterization of a gene responsible for inhibiting propagation of methylated DNA sequences in mcrB1 Escherichia coli strains. J Bacteriol 173: 4707–4716.
Kelleher JE, Raleigh EA (1992) A novel activity in Escherichia coli K-12 that directs restriction of DNA modified at CG dinucleotides. J Bacteriol 173: 5220–5223.
Heitman J, Model P (1987) Site-specific methylases induce the SOS DNA repair response in Escherichia coli. J Bacteriol 169: 3243–3250.
Bickle T (1982) The ATP dependent restriction endonucleases, pp. 85–108, In: Linn SM, Roberts RJ (ed) Nucleases Cold Spring Harbor, New York, NY: Cold Spring Harbor Laboratory.
Raleigh EA, Murray NE, Revel H, Blumenthal RM, Westaway D, Reith AD, Rigby PWJ, Elhai J, Hanahan D (1988) McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning. Nucl Acids Res 16: 1563–1575.
Woodcock DM, Crowther PJ, Doherty J, Jefferson S, DeCruz E, Noyer-Weidner M, Smith SS, Michael MZ, Graham MW (1989) Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucl Acids Res 17: 3469–3478.
Kohler SW, Provost GS, Kretz PL, Dycaico MJ, Sorge JA, Short JM (1990) Development of a short-term, in vivo mutagenesis assay: The effects of methylation on the recovery of a lambda phage shuttle vector from transgenic mice. Nucl Acids Res 18: 3007–3013.
Kretz PL, Reid CH, Greener A, Short JM (1989) Effect of lambda packaging extract mcr restriction activity on DNA cloning. Nucl Acids Res 17: 5409.
Kretz PL, Short JM (1989) Gigapack™ II: Restriction Free (HsdR, McrA, McrB, Mrr) Lambda Packaging Extracts. Strategies 2: 25–26.
Clark AJ, Low KB (1988) The Recombination of Genetic Material. Academic Press, New York, NY.
John Maliyakal E (1992) An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucl Acids Res 20: 2381.
Jerpseth BJ, Greener A, Short JM, Viola J, Kretz PL (1993) New restriction-minus derivatives of XLl-Blue E. coli cells. Strategies 6: 24.
Jerpseth BJ, Greener A, Short JM, Viola J, Kretz PL (1993) XLl-Blue MRF E. coli cells: McrA ―, McrCB ―, McrF ―, Mrr ―, HsdR ― derative of XLl-Blue cells. Strategies 5: 81–83.
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© 1994 Springer Science+Business Media Dordrecht
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Snead, M.A., Kretz, P.L., Short, J.M. (1994). Methods for generating plant genomic libraries. In: Gelvin, S.B., Schilperoort, R.A. (eds) Plant Molecular Biology Manual. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0511-8_24
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DOI: https://doi.org/10.1007/978-94-011-0511-8_24
Publisher Name: Springer, Dordrecht
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