Summary
An enzyme, galactosyltransferase, able to catalyze the formation of galactose polymers was detected in cell-free extracts of a wild type strain of Neurospora crassa. Enzyme activity was found in both the supernatant and the particle fractions after centrifugation at 100,000 xg. The enzyme assayed in the 100,000 xg supernatant showed a 4fold difference in specific activity as compared to that found in the particle fraction.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
N. C. Mishra, in: Advances in Genetics, Vol. 19. Ed. E. W. Caspari. Academic Press, New York 1977.
E. L. Tatum, Naturw. Rdsch. Stuttg.26, 140 (1973).
T. M. Harold, Biochem. biophys. Acta57, 59 (1972).
P. R. Mahadevan and E. L. Tatum, J. Bact.90, 1073 (1965).
J. L. Reissig and J. E. Glasgow, J. Bact.106, 882 (1971).
J. B. Rand, Ph. D. thesis, The Rockefeller University, New York 1975.
H. J. Vogel, Am. Nat.98, 435 (1964).
O. H. Lowry, N. H. Rosebrough, A. L. Farr and R. J. Randall, J. biol. Chem.193, 265 (1951).
I. Smith, Chromatographic and Electrophoretic Techniques, vol. 1 p. 246. Ed. Interscience Publishers, Inc., New York 1960.
D. K. Podolsky, M. M. Weiser, J. T. LaMont and K. J. Issclbacher, Proc. nat. Acad. Sci. US,71, 904 (1974).
H. F. Terenzi, M. M. Flawia and H. N. Torres, Neurospora News Letter21, 19 (1976).
Author information
Authors and Affiliations
Additional information
Acknowledgment. This work was supported by a grant from the Research Corporation.
Rights and permissions
About this article
Cite this article
Forsthoefel, A., Mishra, N.C. Galactosyltransferase of Neurospora. Experientia 33, 864–865 (1977). https://doi.org/10.1007/BF01951247
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01951247