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Single-nucleotide polymorphisms in porcine mannan-binding lectin A

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Abstract

The MBL1 and MBL2 genes encode mannan-binding lectins (MBL) A and C, respectively, that are collagenous lectins (collectins) produced mainly by the liver. Several single-nucleotide polymorphisms (SNPs) in the human MBL2 gene are responsible for various innate immune dysfunctions due to abnormal structure or expression of human MBL-C. The MBL1 gene encodes MBL-A, which has bacteria-binding properties in pigs and rodents but is mutated to a pseudogene in humans and chimpanzees. In these studies, we surveyed both porcine MBL genes for SNPs that might impair disease resistance. Single-strand conformational polymorphism (SSCP) analysis of MBL cDNAs from porcine liver revealed three SNPs within the coding region of MBL1 in various breeds of pigs. One nonsynonymous SNP that substituted cysteine for glycine in the collagen-like domain of pig MBL-A was found by a multiplex PCR test in all European pig breeds examined, with allele frequencies ranging from 1.4 to 46.4%. No SNPs were identified in the coding region of porcine MBL2 but the expression of MBL-C in the liver was widely variable in comparison to the expression of MBL-A, GAPDH, PigMAP, and haptoglobin. These results indicate that some pigs have a miscoding defect in MBL-A and a possible expression defect in MBL-C, which are analogous to coding and promoter polymorphisms that affect human MBL-C.

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Abbreviations

MBL:

mannan-binding lectin

SNP:

single-nucleotide polymorphism

SSCP:

single-strand conformational polymorphism

MASP:

MBL-associated serine protease

sMAP:

small MBL-associated protein

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

PigMAP:

pig major acute-phase protein

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Acknowledgments

We appreciate the assistance of Andrew Brooks, Janet MacInnes, Mario Jacques, Bette Anne Quinn, Yanping Lou, John Peacock, Lilly Bojarski, and Alex Loretti. The pathologists at the Animal Health Laboratory, especially Josepha Delay and Beverly McEwen, as well as Robert Friendship, were instrumental in the collection of the diseased pig tissue samples. Funding from the Canadian Research Network on Bacterial Pathogens of Swine, the Natural Sciences and Engineering Research Council of Canada (NSERC), Ontario Pork, the Ontario Ministry of Agriculture and Food, and the Ontario Veterinary College supported this work.

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Authors and Affiliations

  1. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Brandon N. Lillie, Natalie D. Keirstead & M. Anthony Hayes

  2. Department of Animal and Poultry Science, University of Guelph, Guelph, ON, N1G 2W1, Canada

    E. James Squires

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  1. Brandon N. Lillie
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  2. Natalie D. Keirstead
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  3. E. James Squires
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  4. M. Anthony Hayes
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Correspondence to M. Anthony Hayes.

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Lillie, B.N., Keirstead, N.D., Squires, E.J. et al. Single-nucleotide polymorphisms in porcine mannan-binding lectin A. Immunogenetics 58, 983–993 (2006). https://doi.org/10.1007/s00251-006-0160-z

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  • DOI: https://doi.org/10.1007/s00251-006-0160-z

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