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Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera

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Abstract

Antigens present in aqueous n-butanolic extracts (BE) of Schistosoma mansoni (Venezuelan JL strain), Schistosoma intercalatum (Cameroon EDEA strain), and Schistosoma haematobium (Yemen strain) adult worm membranes were compared in immunoblot against sera of patients infected with S. mansoni, S. intercalatum, S. haematobium, Schistosoma japonicum, or Schistosoma mekongi looking for similarities (common antigens) and differences (species-specific antigens). About 17 S. mansoni BE polypeptides (M r ∼8 to >80 kDa) were commonly recognized by S. mansoni-infected patient sera from Venezuela, Senegal, and Ethiopia. S. intercalatum-, S. haematobium-, or S. japonicum-infected sera were almost unreactive with S. mansoni BE. Nonetheless, S. mekongi-infected sera weakly cross-reacted with a ∼10–15-kDa subset of S. mansoni BE. About 72.7% of S. intercalatum-infected patient sera reacted with a ∼19–21-kDa complex in S. intercalatum BE and cross-reacted with a similar complex in S. haematobium BE. Conversely, all S. haematobium-infected patient sera reacted with a ∼19–21-kDa complex in S. haematobium BE and cross-reacted with the ∼19–21-kDa complex in S. intercalatum BE; S. mansoni- and S. japonicum-infected patient sera did not react with S. intercalatum or S. haematobium BE. Results showed the presence of a common membrane antigen between African schistosome species and species-specific antigens in S. mansoni BE that could be useful to discriminate between species and/or to detect Schistosoma infections.

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Acknowledgements

We thank the CNRS (France)-FONACIT (Venezuela) convention for financing the travels between France and Venezuela and ECOS-Nord V06A01. We thank Cesar Matos and Anne Rognon for their contribution to maintenance of life cycles of the schistosome species.

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Authors and Affiliations

  1. Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (IVIC), Apdo. 21827, Caracas, 1020 A, Venezuela

    Italo M. Cesari, Diana E. Ballen & L. Mendoza

  2. Laboratoire de Parasitologie, Mycologie et Médecine Tropicale, U.F.R. de Médecine, 2bis, boulevard Tonnellé, 37032, Tours cedex, France

    Alain Ferrer

  3. Département de Parasitologie–Mycologie–Médecine Tropicale Faculté de Médecine et des Sciences de la Santé, Université des Sciences de la Santé, B.P. 4009, Libreville, Gabon

    Maryvonne Kombila & Dominique Richard-Lenoble

  4. UMR 5244 CNRS-EPHE-UPVD Biologie et Ecologie Tropicale et Méditerranéenne, Université de Perpignan Via Domitia, 52, Ave Paul Alduy, 66860, Perpignan Cedex, France

    Jean-Pierre Pointier & Andre Théron

Authors
  1. Italo M. Cesari
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  2. Diana E. Ballen
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  3. L. Mendoza
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  4. Alain Ferrer
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  5. Jean-Pierre Pointier
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  6. Maryvonne Kombila
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  7. Dominique Richard-Lenoble
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  8. Andre Théron
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Correspondence to Andre Théron.

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Cesari, I.M., Ballen, D.E., Mendoza, L. et al. Immunoblot analysis of membrane antigens of Schistosoma mansoni, Schistosoma intercalatum, and Schistosoma haematobium against Schistosoma-infected patient sera. Parasitol Res 106, 1225–1231 (2010). https://doi.org/10.1007/s00436-010-1798-x

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  • DOI: https://doi.org/10.1007/s00436-010-1798-x

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