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The genistein stimulon of Bradyrhizobium japonicum

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Abstract

An initializing step in the rhizobia–legume symbiosis is the secretion of flavonoids by plants that leads to the expression of nodulation genes in rhizobia. Here we report the genome-wide transcriptional response of Bradyrhizobium japonicum to genistein, an isoflavone secreted by soybean. About 100 genes were induced in the wild type. This included all nod box-associated genes, the flagellar cluster and several genes that are likely to be involved in transport processes. To elucidate the role of known regulators, we analysed mutant strains. This revealed that the two-component response regulator NodW is essential for induction of almost all genistein-inducible genes, with the exception of 8 genes. The phenotype of the nodW mutant could be partially suppressed by overexpression of NwsB, which is also a two-component response regulator. These data indicate that genistein has a much broader function than mere induction of nod genes.

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Acknowledgments

We are grateful to H. Hennecke, H-M. Fischer and S. Zehner for valuable comments on the manuscript and for discussion. This work was supported by the Bundesministerium für Bildung und Forschung as part of the program GenoMik.

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Authors and Affiliations

  1. Institute of Genetics, Dresden University of Technology, Helmholtzstraße 10, 01069, Dresden, Germany

    Kathrin Lang & Michael Göttfert

  2. Institute of Microbiology, Swiss Federal Institute of Technology Zurich, Wolfgang-Pauli-Strasse 10, 8093, Zurich, Switzerland

    Andrea Lindemann & Felix Hauser

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  1. Kathrin Lang
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  2. Andrea Lindemann
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  3. Felix Hauser
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  4. Michael Göttfert
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Correspondence to Michael Göttfert.

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Communicated by A. Hirsch.

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Lang, K., Lindemann, A., Hauser, F. et al. The genistein stimulon of Bradyrhizobium japonicum . Mol Genet Genomics 279, 203–211 (2008). https://doi.org/10.1007/s00438-007-0280-7

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