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Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes

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Abstract

Worldwide, a variety of pathogens negatively affect potato production, resulting in an estimated 22% annual yield reduction. Wild Solanum species represent a unique gene pool where all the traits necessary to improve the cultivated potato can be found. Therefore, breeding efforts for improved disease resistance and research aimed at characterizing wild germplasm have been extensively made. In this paper, sources of resistance to Phytophthora infestans, Erwinia carotovora subsp. carotovora, Fusarium solani and Globodera spp. have been investigated in several clones of two Solanum species originating from Central Mexico (S. bulbocastanum and S. cardiophyllum). Interestingly, we found sources of combined resistance to late blight and bacterial soft rot. This is an important finding considering that the development of resistant potato varieties has been hindered by the scarcity of resistant germplasm. In addition, we explored molecular differences within and between the two species generating AFLP fingerprints. By means of six primer pair combinations, we found 13 and 16 putative species-specific AFLP markers for S. bulbocastanum and S. cardiophyllum, respectively, and a bounty of markers useful for mapping, MAS, and cloning purposes. The phenotypic and molecular information associated to S. bulbocastanum and S. cardiophyllum for designing strategies of assisted selection are discussed.

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Acknowledgments

Contribution no. 127 from Department of Soil, Plant and Environmental Science. This research was carried out within the project “Caratterizzazione ed utilizzazione di specie selvatiche diploidi di patata originarie del Sud America” founded by Italian Ministry of Agriculture (MiPAF).

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Authors and Affiliations

  1. Department of Soil, Plant and Environmental Sciences, University of Naples “Federico II”, Via Università 100, Portici, 80055, Italy

    Riccardo Aversano, Maria Raffaella Ercolano, Luigi Frusciante, Luigi Monti & Domenico Carputo

  2. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall/1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    James M. Bradeen

  3. Istituto di Patologia Vegetale, University of Naples “Federico II”, Via Università 100, Portici, 80055, Italy

    Gennaro Cristinzio & Astolfo Zoina

  4. Istituto per la Protezione delle Piante, Sezione di Bari, C.N.R., Via G. Amendola 165/A, Bari, 70126, Italy

    Nicola Greco

  5. C.R.A. – Istituto Sperimentale per la Patologia Vegetale, Via C.G. Bertero 22, Roma, 00156, Italy

    Salvatore Vitale

Authors
  1. Riccardo Aversano
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  2. Maria Raffaella Ercolano
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  9. Salvatore Vitale
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  10. Domenico Carputo
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Correspondence to Domenico Carputo.

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Aversano, R., Ercolano, M.R., Frusciante, L. et al. Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes. Genet Resour Crop Evol 54, 1797–1806 (2007). https://doi.org/10.1007/s10722-006-9201-6

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  • DOI: https://doi.org/10.1007/s10722-006-9201-6

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