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Development of Sensitive, Rapid, and Effective Immunoassays for the Detection of Vitamin B12 in Fortified Food and Nutritional Supplements

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Abstract

An indirect competitive enzyme-linked immunosorbent assay (Ic-ELISA) method and lateral-flow immunochromatographic (ICA) strip assay method were developed for the detection of vitamin B12 (four major forms, cyanocobalamin, hydroxocobalamin, adenosylcobalamin, and methylcobalamin) in different food products. The limit of detection and 50 % inhibitory concentration for the Ic-ELISA method were 0.065 and 0.43 ng/mL, respectively. The visual limit of detection and cutoff value for the lateral-flow ICA strip assay method were 1 and 4 ng/mL, respectively. For the detection of fortified food and nutritional supplements in vitamin tablets, energy drink, and infant milk powder samples, the recovery rates were in the range of 81 to 122 % for the Ic-ELISA method, and even the lowest content in infant milk powder was identified by the lateral-flow ICA strip assay. Therefore, both of these methods are sensitive, rapid, and effective and are suitable for the on-site detection and rapid screening of mass samples.

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Acknowledgments

This work is financially supported by the Key Programs from MOST (2012AA06A303 and 2012BAD29B04).

Author Contributions

Hua Kuang and Chuanlai Xu had the original idea for the study, and with all co-authors carried out the design. Chuanlai Xu was responsible for the recruitment and follow-up of study participants. Dezhao Kong, Liqiang Liu, and Shanshan Song were responsible for the data cleaning, and Dezhao Kong carried out the analyses. Dezhao Kong and Liqiang Liu drafted the manuscript, which was revised by Chuanlai Xu. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, People’s Republic of China

    Dezhao Kong, Liqiang Liu, Shanshan Song, Hua Kuang & Chuanlai Xu

  2. School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, 214122, People’s Republic of China

    Dezhao Kong, Liqiang Liu, Shanshan Song, Hua Kuang & Chuanlai Xu

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  1. Dezhao Kong
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Correspondence to Chuanlai Xu.

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Shanshan Song and Hua Kuang declare that they got grant from MOST (2012AA06A303). Chuanlai Xu declares that he got grant from MOST (2012BAD29B04).

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Dezhao Kong, Liqiang Liu, Shanshan Song, and Hua Kuang declare no conflict of interest.

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This article does not contain any studies involving human subjects. All animal studies were carried out under the guidance of animal welfare committee of Jiangnan University.

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Kong, D., Liu, L., Song, S. et al. Development of Sensitive, Rapid, and Effective Immunoassays for the Detection of Vitamin B12 in Fortified Food and Nutritional Supplements. Food Anal. Methods 10, 10–18 (2017). https://doi.org/10.1007/s12161-016-0543-1

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