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Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus

  • Virology
  • Published:
Journal of Microbiology

Abstract

Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172 cases reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RT-LAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.

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Acknowledgments

The present study was supported by a grant from the Next-Generation BioGreen21 Program (grant no. PJ011835), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea

    Jae Woong Lee, Yu-Jung Won, Lae Hyung Kang & Soon-Young Paik

  2. Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54596, Republic of Korea

    Sung-Geun Lee

  3. Department of Biotechnology, Daegu Catholic University, Gyeongsan-si, 38430, Republic of Korea

    Seung-Won Park

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  1. Jae Woong Lee
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Correspondence to Soon-Young Paik.

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Lee, J.W., Won, YJ., Kang, L.H. et al. Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus. J Microbiol. 58, 711–715 (2020). https://doi.org/10.1007/s12275-020-0109-1

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  • DOI: https://doi.org/10.1007/s12275-020-0109-1

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