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Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla

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Abstract

Spectral quality is an important factor for in vitro development of explants in a bioreactor system. Based on the need to optimize micropropagation for E. grandis × E. urophylla clones, the aim of the study was to assess the spectral quality of in vitro multiplication in temporary immersion bioreactor (TIB). The tissue used to generate the explants (i.e., the nodal segment with 1 cm of length and two axillary bud without leaves) was previously in vitro established and multiplied, it derived from ministumps of E. grandis × E. urophylla clone grown in a semi-hydroponic system. The spectral quality of in vitro multiplication was assessed through five light sources (i.e., fluorescent lamp, red, green, blue, and yellow cellophane). Morphological and anatomical features of tissues grown in TIB were evaluated at 90 days. Based on the results, yellow and blue spectral qualities were the most suitable to be adopted for in vitro multiplication of E. grandis × E. urophylla, since they enabled lesser hyperhydricity, favors high number of shoots per explant and shoot length, as well as thicker mesophyll and spongy parenchyma; arise as an alternative for large-scale production of eucalypts clonal plants.

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Acknowledgements

We thank the National Counsel of Technological and Scientific Development (CNPq), Coordination for Improvement of Higher Education Personnel (CAPES) and Research Support Foundation of the State of Minas Gerais (FAPEMIG) for the financial support and scholarship.

Funding

This work was carried out with the support of the Coordination for the Improvement of Higher Education Personnel—Brazil (CAPES), the National Council for Scientific and Technological Development (CNPq) and the Research Support Foundation of the State of Minas (FAPEMIG).

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Authors and Affiliations

  1. Department of Forestry Sciences, Federal University of Lavras, P.O.B. 3037, Lavras, MG, 37200-900, Brazil

    Denys Matheus Santana Costa Souza, Maria Lopes Martins Avelar, Sérgio Bruno Fernandes, Letícia Vaz Molinari & Gilvano Ebling Brondani

  2. Coordination of Natural Sciences, Federal University of Maranhão, Codó Campus, Av. Dr. José Anselmo, 2008, São Benedito, Codó, Maranhão, 65.400-000, Brazil

    Eduardo Oliveira Silva

  3. Department of Botany, Federal University of Lavras, P.O.B. 3037, Lavras, Minas Gerais, 37200-900, Brazil

    Vinícius Politi Duarte

Authors
  1. Denys Matheus Santana Costa Souza
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  2. Maria Lopes Martins Avelar
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  3. Sérgio Bruno Fernandes
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  4. Eduardo Oliveira Silva
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  5. Vinícius Politi Duarte
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  6. Letícia Vaz Molinari
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  7. Gilvano Ebling Brondani
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Contributions

Study group: M.Sc. DMSCS—conduction of experiment, analyses, review, discussion of the theme; M.Sc. SBF—conduction of experiment, analyses, review, discussion of the theme; M.Sc. MLMA—conduction of experiment, analyses, review, discussion of the theme; M.Sc. LVM—analyses, discussion of the theme; Dr. ESVPD conduction of experiment, review, discussion of the theme; Dr. GEB—Supervisor, analyses, review, discussion of the theme.

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Correspondence to Denys Matheus Santana Costa Souza.

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Souza, D.M.S.C., Avelar, M.L.M., Fernandes, S.B. et al. Spectral quality and temporary immersion bioreactor for in vitro multiplication of Eucalytpus grandis × Eucalyptus urophylla. 3 Biotech 10, 457 (2020). https://doi.org/10.1007/s13205-020-02447-3

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