Abstract
Purpose To determine the effects of age on blood flow measurements obtained using the scanning laser Doppler flowmeter (SLDF).
Method Using SLDF (Heidelberg retina flowmeter, Heidelberg Engineering, Germany) three 10° images were taken of the superior temporal retina and three further images of the superior temporal neuroretinal rim in 15 young, healthy subjects (mean age 27.9 years ± 6.2 years) and 15 mature, healthy subjects (mean age 65.2 years ± 13.7 years). In addition, measurements were taken of the lamina cribrosa in 12 of the volunteers from each subject group (mean age 27.1 ± 6.3 years and 64.8 ± 13.2 years respectively). Using a 10 × 10 pixel measurement frame, blood flow readings were obtained at a predetermined position on the retina, neuroretinal rim and lamina cribrosa. Student’s two-tailed unpaired t-tests were used to compare measures of blood flow, volume and velocity between the two subject groups (P < 0.05). In addition, linear regression analysis was used to assess the relationship between age and blood flow, volume and velocity at the retina, neuroretinal rim and lamina cribrosa.
Results Retinal blood volume measured at the retina was significantly lower in the mature compared with the young subject group (P = 0.01). Mature subjects also exhibited reduced blood flow and velocity at the neuroretinal rim (P = 0.01 for both parameters) and lamina cribrosa (P = 0.008 and P = 0.01 respectively). Regression analysis revealed negative trends for all blood flow parameters in each of the anatomical areas with advancing age. Significant negative correlations were obtained for retinal blood volume (r = −0.455, P < 0.05), neuroretinal rim blood velocity (r = −0.359, P < 0.05) and lamina cribrosa blood volume (r = −0.475, P < 0.05).
Conclusion Capillary blood flow in the retina, neuroretinal rim and lamina cribrosa decreases with advancing age. This may be of consequence in the progression of chronic ocular diseases such as glaucoma, and should be considered in the longitudinal determination of change in disease monitoring.
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References
Michelson G, Langhans M, Groh J . Perfusion of the juxtapapillary retina and the neuroretinal rim area in primary open angle glaucoma. J Glaucoma 1996; 5: 91–98
Michelson G, Schmauss B . Two dimensional mapping of the perfusion of the retina and optic nerve head. Br J Ophthalmol 1995; 79: 1126–1132
Bonner R, Nossal R . Model for laser Doppler measurements of blood flow in tissue. Appl Optics 1981; 20: 2097–2107
Chauhan B, Smith F . Confocal scanning laser Doppler flowmetry: experiments in a model flow system. J Glaucoma 1997; 6: 237–245
Strenn K et al. Reproducibility and sensitivity of scanning laser Doppler flowmetry during graded changes in PO2 . Br J Ophthalmol 1997; 81: 360–364
Michelson G et al. Principle, validity, and reliability of scanning laser Doppler flowmetry. J Glaucoma 1996; 5: 99–105
Nicolela M et al. Reproducibility of retinal and optic nerve head blood flow measurements with scanning laser Doppler flowmetry. J Glaucoma 1997; 6: 157–164
Findl O et al. Ocular haemodynamics and colour contrast sensitivity in patients with type 1 diabetes. Br J Ophthalmol 2000; 84: 493–498
Remsch H, Lang GE, Lang GK . Changes of capillary blood flow in age related macular degeneration. Invest Ophthalmol Vis Sci 1999; 40: S486
Holló G et al. Evaluation of the peripapillary circulation in healthy and glaucoma eyes with scanning laser Doppler flowmetry. Int Ophthalmol 1997; 20: 71–77
Nicolela M, Hnik P, Drance S . Scanning laser Doppler flowmeter study of retinal and optic disk blood flow in glaucomatous patients. Am J Ophthalmol 1996; 122: 775–783
Hayreh S . The role of age and cardiovascular disease in glaucomatous optic neuropathy. Surv Ophthalmol 1999; 43: S27–S42
Garway-Heath D, Wollstein G, Hitchings R . Aging changes of the optic nerve head in relation to open angle glaucoma. Br J Ophthalmol 1997; 81: 840–845
Chung H et al. Peripapillary retinal blood flow in normal tension glaucoma. Br J Ophthalmol 1999; 83: 466–469
Ravalico G et al. Age related ocular blood flow changes. Invest Ophthalmol Visual Sci 1996; 37: 2645–2650
Massey A, Geyer O, Silver DM . The effect of eye volume and age on pulsatile ocular blood flow. Invest Opthalmol Vis Sci 1999; 40: S490
Groh M et al. Influence of age on retinal and optic nerve head circulation. Ophthalmology 1996; 103: 529–534
Neetens A . Vascular supply of the optic nerve head. Neuroophthalmology 1994; 14: 113–120
Buchi ER . The blood supply to the optic nerve head In: Kaiser HJ, Flammer J, Hendrickson P (eds) Ocular Blood Flow: New Insights into the Pathogenesis of Ocular Diseases Karger: Basel 1996 pp 1–8
Bill A . Ocular circulation In: Moses RA (ed) Adlers Physiology of the Eye Mosby: St Louis 1981 184–203
Wadsworth RM . Calcium and vascular reactivity in ageing and hypertension. J Hypertens 1990; 8: 975–983
Wei JY . Age and the cardiovascular system. N Engl J Med 1992; 327: 1735–1739
Hayreh SS . Pathogenosis of optic nerve damage and visual field defects In: Heilman LK, Richardson KT, (eds) Glaucoma: Conceptions of a Disease Georg Thieme: Stuttgart 1978 pp 104–107
Albon J et al. Changes in the collagenous matrix of the ageing human lamina cribosa. Br J Ophthalmol 1995; 79: 368–375
Albon J et al. Age related compliance of the lamina cribosa in human eyes. Br J Ophthalmol 2000; 84: 318–323
Bohdanecka Z et al. Influence of acquisition parameters on hemodynamic measurements with the Heidelberg retinal flowmeter at the optic disc. J Glaucoma 1998; 7: 151–157
Hosking SL et al. Detector sensitivity influences blood flow sampling in scanning laser Doppler flowmetry. Graefes Archiv Clin Exp Ophthalmol 2001; 239: 407–410
Dallinger S et al. Age dependence of choroidal blood flow. J Am Geriatr Soc 1998; 46: 484–487
Grunwald JE, Hariprasad SM, DuPont J . Effects of aging on foveolar choroidal circulation. Arch Ophthalmol 1998; 116: 150–154
Zeimer RC . Circadian variations in intraocular pressure. In: Ritch R, Shields MB, Krupin T (eds). The Glaucomas CV Mosby: St Louis 1989
Harris A et al. Aging affects the retrobulbar circulation differently in women and men. Arch Ophthalmol 2000; 118: 1076–1080
Straubhaar M et al. Chroidal laser Doppler flowmetry in healthy subjects. Arch Ophthalmol 2000; 118: 211–215
James C . POBF [editorial]. Br J Ophthalmol 1998; 82: 720–721
Jonas JB, Nguyen NX, Naumann GO . The retinal nerve fiber layer in normal eyes. Ophthalmology 1989; 96: 627–632
Jonas JB et al. Human optic nerve fiber count and optic disc size. Invest Ophthalmol Vis Sci 1992; 33: 2012–2018
Gao H, Hollyfield JG . Aging of the human retina: differential loss at neurons and retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 1992; 33: 1–17
Lee WR, Blass GE, Shaw DC . Age-related retinal vasculopathy. Eye 1987; 1: 296–303
Hayreh SS, Bill A, Sperber GO . Effects of high intraocular pressure on the glucose metabolism in the retina and optic nerve in bold atherosclerotic monkeys. Graefes Arch Clin Exp Ophthalmol 1994; 232: 745–752
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Embleton, S., Hosking, S., Roff Hilton, E. et al. Effect of senescence on ocular blood flow in the retina, neuroretinal rim and lamina cribrosa, using scanning laser Doppler flowmetry. Eye 16, 156–162 (2002). https://doi.org/10.1038/sj.eye.6700100
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DOI: https://doi.org/10.1038/sj.eye.6700100