Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags

Ayasa Nagatani; Kosuke Minamihata; Motoyasu Adachi; Rie Wakabayashi; Masahiro Goto; Noriho Kamiya

doi:10.1039/D4RA08326A

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Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags†

Ayasa Nagatani,

^a Kosuke Minamihata,*^a Motoyasu Adachi,

^bc Rie Wakabayashi,

^a Masahiro Goto

^ad and Noriho Kamiya

^ad

Author affiliations

* Corresponding authors

^a Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan

^b Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan

^c Department of Quantum Life Science, Graduate School of Science, Chiba University, Chiba, Japan

^d Division of Biotechnology, Center for Future Chemistry, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan

Abstract

We have achieved a novel co-crystal in which the dual functions of the protein and single-stranded DNA are maintained by introducing a charged peptide tag at the C-terminus of the protein. The functionalities allowed the co-crystals to be modified with high selectivity. Additionally, we have confirmed that energy transfer occurs between the two molecules modified within the co-crystal. Therefore, this co-crystal has the potential as a novel biomaterial applicable to biosensors.

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DOI: https://doi.org/10.1039/D4RA08326A
Article type: Paper
Submitted: 24 Nov 2024
Accepted: 17 Feb 2025
First published: 03 Mar 2025
This article is Open Access

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RSC Adv., 2025,15, 6817-6822

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Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags

A. Nagatani, K. Minamihata, M. Adachi, R. Wakabayashi, M. Goto and N. Kamiya, RSC Adv., 2025, 15, 6817 DOI: 10.1039/D4RA08326A

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Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags†

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Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags

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