Steroid Receptor Isoform Expression in Drosophila Nociceptor Neurons Is Required for Normal Dendritic Arbor and Sensitivity
Fig 3
Ecdysone receptor mutants are less sensitive to noxious mechanical stimulation.
Foraging third instar larvae were stimulated on their dorsal surface with a von Frey filament calibrated to deliver 45 mN. Larvae responding with a nocifensive roll were classified as responders. Distributions were compared with Fisher’s exact test. Asterisks indicate statistically different groups (* is p<0.05, *** is p<0.001). N > 90. (A) Mutants bearing one null allele of EcR (EcRM554fs) and one temperature sensitive allele (EcRA483T) were significantly less sensitive than controls comprising the genetic backgrounds of each mutation (+ = Canton-S). (B) EcR RNAi is driven by the mifepristone-inducible neuron-specific elav-Geneswitch. (C-F) Various EcR and a USP RNAi are driven by ppk1.9-Gal4, specific to the nociceptive class IV multidendritic neurons.
