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Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation

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Antibody Engineering Protocols

Part of the book series: Methods In Molecular Medicine™ ((MIMB,volume 51))

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Abstract

The application of phage display to the screening of antibody V-gene libraries promises to replace animal immunization and hybridoma technology as a means of antibody generation (1,2). Synthetic libraries offer the potential for expanding the immune system repertoire and designing libraries for particular sets of antigens. We have developed a method for the generation of synthetic antibody gene libraries by which spiked oligonucleotides are assembled by a ligase chain reaction protocol (3). This is a synthetic adaptation of a reaction that was previously used as a diagnostic tool (4,5).

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Author information

Authors and Affiliations

  1. Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada

    Su-jun Deng, C. Roger MacKenzie & Saran A. Narang

Authors
  1. Su-jun Deng
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  2. C. Roger MacKenzie
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  3. Saran A. Narang
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Editor information

Editors and Affiliations

  1. Department of Anesthesiology, University of Nebraska Medical Center, Omaha, NE

    Sudhir Paul

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© 1995 Humana Press Inc.

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Deng, Sj., MacKenzie, C.R., Narang, S.A. (1995). Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation. In: Paul, S. (eds) Antibody Engineering Protocols. Methods In Molecular Medicine™, vol 51. Humana Press. https://doi.org/10.1385/0-89603-275-2:329

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  • DOI: https://doi.org/10.1385/0-89603-275-2:329

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-275-0

  • Online ISBN: 978-1-59259-538-9

  • eBook Packages: Springer Protocols

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