Abstract
Laboratories like the American Type Culture Collection (Rockville, MD) have been using freeze-drying or lyophilization techniques as a means of preserving microorganisms for more than 50 years. There are numerous variations of the method, and elaborate equipment is available to improve environmental control and to allow large numbers of samples to be processed.
Similar content being viewed by others
References
Kirsop, B. E. and Snell, J. J. S (1984) Mauztenance of Mzcroorganlsms. Academic, Orlando, FL.
Gherna, R. L. (1994) Culture Preservation, in Methods for General and Molecular Bactew1ogy (Gerhardt, P., Murray, R. G. E, Wood, W A., and Krieg, N. R., eds.), American Soctety for Mwrobiology, Washmgton, DC, pp. 278–292.
Janda, I. and Opekarova, M. (1989) Long-term preservation of active luminous bacteria by lyophilization. J Biolum. Chemilum 3, 27–29.
Bulich, A. A. (1982) A practical and reliable method for monitoring the toxicity of aquatic samples. Process Biochem. 17, 45–47
Van Dyk, T. K., Majarran, W. R., Konstantinov, K. B., Young, R. M., Dhurjati, P. S., and LaRossa, R. (1994) Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions Appl. Environ Microbial. 60, 1414–1420.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (eds.) (1989) Molecular Cloning A Laboratory Manual, 2nd ed., vol. 3, appendix A. 1 Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Rupam, S P., Gu, M. B., Konstantinov, K. B., Dhurjati, P S., Van Dyk, T. K., and LaRossa, R. A. (1996) Characterizatton of the stress response of a bioluminescent biological sensor in batch and continuous cultures. Biotechnol. Prog. 12, 387–392.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Wagner, L.W., Van Dyk, T.K. (1998). Cryopreservation and Reawakening. In: LaRossa, R.A. (eds) Bioluminescence Methods and Protocols. Methods in Molecular Biology™, vol 102. Humana Press. https://doi.org/10.1385/0-89603-520-4:123
Download citation
DOI: https://doi.org/10.1385/0-89603-520-4:123
Publisher Name: Humana Press
Print ISBN: 978-0-89603-520-1
Online ISBN: 978-1-59259-577-8
eBook Packages: Springer Protocols